NR-2742_单克隆抗流感病毒H5血凝素(HA)蛋白(VN04-13),A/Vietnam/1203/2004(H5N1),(腹水,小鼠)(单克隆抗体)
产品图片
货号/SKU
NR-2742
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-2742?? Monoclonal Anti-Influenza Virus H5 Hemagglutinin (HA) Protein (VN04-13), A/Vietnam/1203/2004 (H5N1), (ascites, Mouse)(Monoclonal Antibodies)|Influenza A virus|Monoclonal Anti-Influenza Virus H5 Hemagglutinin (HA) Protein (VN04-13), A/Vietnam/1203/2004 (H5N1), (ascites, Mouse)|-20°C to -40°C|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Influenza Virus H5 Hemagglutinin (HA) Protein (VN04-13), A/Vietnam/1203/2004 (H5N1), (ascites, Mouse), NR-2742."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Antibody Class: IgG2aκ
Mouse monoclonal antibody specific to a recombinant form of the H5 hemagglutinin (HA) protein (GenPept: AAT73274) of the A/Vietnam/1203/2004 (H5N1) strain of influenza virus was produced in mouse ascites. Ascites formation was induced by injecting cultured hybridoma cells into the peritoneal cavity of BALB/c mice that had been primed with Incomplete Freund's adjuvant. Antibody-rich ascites fluid was aseptically harvested 1 to 2 weeks following hybridoma cell injection. The harvested ascites fluid was pooled and then clarified using centrifugation and filtration. Sodium azide (0.02%) and gentamycin (0.01%) were added to the pooled ascites fluid prior to vialing and lyophilization. NR-2742 is specific for the H5 HA subtype of the influenza A virus as determined in standard hemagglutination inhibition (HI) assays. NR-2742 also demonstrates high reactivity within the H5 HA subtype of recent Asian isolates of influenza A virus based on HI assays.
Each vial of NR-2742 contains lyophilized (1.0 mL containing 0.02% sodium azide and 0.01% gentamycin) mouse ascites fluid.
NR-2742 should be reconstituted with 1.0 mL of sterile distilled water.
Antibody Class: IgG2aκ
Mouse monoclonal antibody specific to a recombinant form of the H5 hemagglutinin (HA) protein (GenPept: AAT73274) of the A/Vietnam/1203/2004 (H5N1) strain of influenza virus was produced in mouse ascites. Ascites formation was induced by injecting cultured hybridoma cells into the peritoneal cavity of BALB/c mice that had been primed with Incomplete Freund's adjuvant. Antibody-rich ascites fluid was aseptically harvested 1 to 2 weeks following hybridoma cell injection. The harvested ascites fluid was pooled and then clarified using centrifugation and filtration. Sodium azide (0.02%) and gentamycin (0.01%) were added to the pooled ascites fluid prior to vialing and lyophilization. NR-2742 is specific for the H5 HA subtype of the influenza A virus as determined in standard hemagglutination inhibition (HI) assays. NR-2742 also demonstrates high reactivity within the H5 HA subtype of recent Asian isolates of influenza A virus based on HI assays.
Each vial of NR-2742 contains lyophilized (1.0 mL containing 0.02% sodium azide and 0.01% gentamycin) mouse ascites fluid.
NR-2742 should be reconstituted with 1.0 mL of sterile distilled water.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID进行批准. 抗体类:IgG2aκ 对鼠单克隆抗体特异于H5 Hemagglutinin(HA)蛋白的重组形式(GENPEPT:AAT73274)在小鼠腹水中生产了A /越南/ 1203/2004(H5N1)流感病毒的菌株.通过将培养的杂交瘤细胞注入伴有不完全弗氏佐剂的Balb / C小鼠的腹膜腔中,诱导腹水形成.在杂交瘤细胞注射后无菌地收获富含抗体的腹水流体1至2周.合并收获的腹水流体,然后使用离心和过滤澄清.在小瓶和冻干之前将叠氮化钠(0.02%)和庆大霉素(0.01%)加入到合并的腹水中. NR-2742特异于流感的HA HA亚型,如标准血凝抑制(HI)测定中确定的病毒. NR-2742还在Hi测定的近期血型流感病毒的HA HA亚型内显示出高反应性. 每个小瓶NR-2742含有冻干(1.0ml含有0.02%叠氮化钠和0.01%的0.01%庆大霉素)鼠标腹水流体. NR-2742应重构1.0毫升无菌蒸馏水.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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