产品描述信息
NR-13307?? Plasmid pMRLB.57 Containing Gene Rv0379 (Protein SecE2) from Mycobacterium tuberculosis for Expression in Mycobacterium smegmatis(Plasmid/Vectors)|Mycobacterium tuberculosis|Plasmid pMRLB.57 Containing Gene Rv0379 (Protein SecE2) from Mycobacterium tuberculosis for Expression in Mycobacterium smegmatis|-20°C or colder|NIH - TB Vaccine Testing and Research Materials ContractAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pMRLB.57 Containing Gene Rv0379 (Protein SecE2) from Mycobacterium tuberculosis for Expression in Mycobacterium smegmatis, NR-13307."|Quantity limit for this item is 2 per year. Orders over this limit will be sent to NIAID for approval before shipment.NR-13307 is a recombinant expression vector containing Mycobacterium tuberculosis gene Rv0379, which encodes a putative transport protein, SecE2. Gene Rv0379 was amplified by PCR and cloned into pVV16 for expression in Mycobacterium smegmatis. The gene was cloned with a signal sequence. The expressed protein is histidine-tagged and has an observed molecular weight of 8 kDa.Note: Plasmid pMRLB.57 contains the gene required for kanamycin resistance. The recommended concentration of kanamycin in culture is 50 ?g/mL.Each vial contains approximately 1 ?g of plasmid DNA in 10 mM Tris-HCl, pH 8.5. Lot 07.EC.05.23 contains approximately 1 ?g of plasmid DNA in 10 mM Tris-HCl, pH 7.5.