NR-722SARS冠状病毒,Spike(S)ProteindeltaTM,杆状病毒重组体(蛋白质）
产品图片
货号/SKU
NR-722
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-722??SARS coronavirus, Spike (S) Protein deltaTM, Recombinant from Baculovirus (Proteins)|SARS coronavirus|Spike (S) Protein deltaTM, Recombinant from Baculovirus |-20°C or colder|NIH Biodefense and Emerging Infections Research Resources RepositoryAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-CoV Spike (S) Protein deltaTM, Recombinant from Baculovirus, NR-722."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-722 is a truncated, glycosylated recombinant form of the SARS-CoV spike (S) external envelope glycoprotein. A 60-amino acid deletion of the transmembrane domain (deltaTM) was made to the C-terminal end of the protein. A baculovirus signal sequence, MPLYKLLNVLWLVAVSNA, was added to the N-terminal end of the protein to enhance expression. The SARS-CoV spike (S) protein deltaTM was produced in Sf9 insect cells using a baculovirus expression vector system and was purified using conventional chromatographic techniques. The baculovirus signal sequence is efficiently removed during translocation of the protein across the membrane. The resulting protein, NR-722, has a molecular weight of approximately 150,000 daltons. The purified protein was demonstrated to be functionally active based on its ability to bind to a recombinant form of its functional receptor, angiotensin I converting enzyme-2.
Each vial contains approximately 1 mg of NR-722 in 2.5 mM sodium phosphate (pH 7.2) and 150 mM NaCl.
NR-722 is a truncated, glycosylated recombinant form of the SARS-CoV spike (S) external envelope glycoprotein. A 60-amino acid deletion of the transmembrane domain (deltaTM) was made to the C-terminal end of the protein. A baculovirus signal sequence, MPLYKLLNVLWLVAVSNA, was added to the N-terminal end of the protein to enhance expression. The SARS-CoV spike (S) protein deltaTM was produced in Sf9 insect cells using a baculovirus expression vector system and was purified using conventional chromatographic techniques. The baculovirus signal sequence is efficiently removed during translocation of the protein across the membrane. The resulting protein, NR-722, has a molecular weight of approximately 150,000 daltons. The purified protein was demonstrated to be functionally active based on its ability to bind to a recombinant form of its functional receptor, angiotensin I converting enzyme-2.
Each vial contains approximately 1 mg of NR-722 in 2.5 mM sodium phosphate (pH 7.2) and 150 mM NaCl.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. NR-722是SARS-COV峰值外壳糖蛋白的截短的糖基化重组形式.将跨膜结构域（Deltatm）的60-氨基酸删除对蛋白质的C-末端进行.将杆状病毒信号序列MpletKllnvlwavsna添加到蛋白质的N-末端以增强表达.使用杆状病毒表达载体系统在SF9昆虫细胞中制备SARS-COV峰值蛋白δ并使用常规的色谱技术纯化.在蛋白质横跨膜的易位期间有效地去除杆状病毒信号序列.所得蛋白质NR-722的分子量约为150,000道尔顿.纯化的蛋白质基于其结合其功能受体的重组形式，血管紧张素I转化酶-2的能力，证明纯化的蛋白质是在功能上活性的. 每个小瓶在2.5 mm中含有约1mg NR-722磷酸钠（pH7.2）和150mM NaCl.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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