产品描述信息
NR-51164?? Staphylococcus aureus Fluorescent Reporter Plasmid pSRFPS1, Recombinant in Staphylococcus aureus (Plasmid/Vectors)|Staphylococcus aureus|Staphylococcus aureus Fluorescent Reporter Plasmid pSRFPS1, Recombinant in Staphylococcus aureus |-60°C or colder|EW TriplettAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Staphylococcus aureus Fluorescent Reporter Plasmid pSRFPS1, Recombinant in Staphylococcus aureus, NR-51164.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders overs this limit will be sent to NIAID for approval before shipment. NR-51164 is a glycerol stock of
Staphylococcus aureus (S. aureus), strain RN4220
containing the DsRed.T3(DNT) red fluorescent protein (RFP) reporter plasmid pSRFPS1,
a derivative of the
Escherichia coli (E. coli) - staphylococcal
shuttle vector pKK30.Plasmid pKK30 is a
member of a set of shuttle vectors that retain stability over a number of
generations
in vitro and
in vivo in
Staphylococcus species without antibiotic selection, making them a
powerful genetic tool.
pKK30 contains the P
sarAP1::
dfrA
trimethoprim resistance cassette, a
blaZ
transcriptional terminator (
blaZTT),
a plasmid single stranded origin (
sso)
required for plasmid replication, a double stranded origin (
dso) required for plasmid maintenance in
the absence of antibiotic selection, a
repF
gene encoding for the
S. aureus
Rep protein and the
E. coli R6K? replication origin. pKK30 lacks the four
predicted USA300-specific open reading frames allowing for use of pKK30 in
non-USA300 isolates.The
complete sequence and vector map of pSRFPS1 are available on the Certificate of
Analysis for NR-51164 lot 70010750 and the vector sequence of pSRFPS1
is available from GenBank: (
MF769791). Each vial contains approximately
0.5 mL of bacterial culture in Tryptic
Soy broth containing 10 ?g/mL trimethoprim supplemented with 25%
glycerol.