NR-51163金黄色葡萄球菌荧光报告质粒pSGFPS1,在金黄色葡萄球菌中重组(质粒/载体）

Quantity limit per order for this item is 1.  This item can be ordered twice a year.  Orders overs this limit will be sent to NIAID for approval before shipment.NR-51163 is a glycerol stock of Staphylococcus aureus (S. aureus), strain RN4220 containing the green fluorescent protein (GFP) reporter plasmid pSGFPS1, a derivative of the Escherichia coli (E. coli) - staphylococcal shuttle vector pKK30.Plasmid pKK30 is a member of a set of shuttle vectors that retain stability over a number of generations in vitro and in vivo in Staphylococcus species without antibiotic selection, making them a powerful genetic tool. pKK30 contains the P_sarAP1::dfrA trimethoprim resistance cassette, a blaZ transcriptional terminator (blaZTT), a plasmid single stranded origin (sso) required for plasmid replication, a double stranded origin (dso) required for plasmid maintenance in the absence of antibiotic selection, a repF gene encoding for the S. aureus Rep protein and the E. coli R6K? replication origin. pKK30 lacks the four predicted USA300-specific open reading frames allowing for use of pKK30 in non-USA300 isolates.The complete sequence and vector map of pSGFPS1 are available on the Certificate of Analysis for NR-51163 lot 70010748 and the vector sequence of pSGFPS1 is also available from GenBank: (MF769790).Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth containing 10 ?g/mL trimethoprim supplemented with 25% glycerol.