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产品图片

货号/SKU
NR-51165
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、2
产品描述信息
NR-51165?? Staphylococcus aureus Fluorescent Reporter Plasmid pSFRFPS1, Recombinant in Staphylococcus aureus (Plasmid/Vectors)|Staphylococcus aureus|Staphylococcus aureus Fluorescent Reporter Plasmid pSFRFPS1, Recombinant in Staphylococcus aureus |-60°C or colder|EW TriplettAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Staphylococcus aureus Fluorescent Reporter Plasmid pSFRFPS1, Recombinant in Staphylococcus aureus, NR-51165.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders overs this limit will be sent to NIAID for approval before shipment.
NR-51165 is a glycerol stock of Staphylococcus aureus (S. aureus), strain RN4220 containing the eqFP650 far-red fluorescent protein (FRFP) reporter plasmid pSFRFPS1, a derivative of the Escherichia coli (E. coli) - staphylococcal shuttle vector pKK30.
Plasmid pKK30 is a member of a set of shuttle vectors that retain stability over a number of generations in vitro and in vivo in Staphylococcus species without antibiotic selection, making them a powerful genetic tool. pKK30 contains the PsarAP1::dfrA trimethoprim resistance cassette, a blaZ transcriptional terminator (blaZTT), a plasmid single stranded origin (sso) required for plasmid replication, a double stranded origin (dso) required for plasmid maintenance in the absence of antibiotic selection, a repF gene encoding for the S. aureus Rep protein and the E. coli R6Kγ replication origin. pKK30 lacks the four predicted USA300-specific open reading frames allowing for use of pKK30 in non-USA300 isolates.
The complete sequence and vector map of pSFRFPS1 are available on the Certificate of Analysis for NR-51165 lot 70010752 and the sequence of pSFRFPS1 is available from GenBank: (MF769789).
Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth containing 10 ?g/mL trimethoprim supplemented with 25% glycerol.
NR-51165 is a glycerol stock of Staphylococcus aureus (S. aureus), strain RN4220 containing the eqFP650 far-red fluorescent protein (FRFP) reporter plasmid pSFRFPS1, a derivative of the Escherichia coli (E. coli) - staphylococcal shuttle vector pKK30.
Plasmid pKK30 is a member of a set of shuttle vectors that retain stability over a number of generations in vitro and in vivo in Staphylococcus species without antibiotic selection, making them a powerful genetic tool. pKK30 contains the PsarAP1::dfrA trimethoprim resistance cassette, a blaZ transcriptional terminator (blaZTT), a plasmid single stranded origin (sso) required for plasmid replication, a double stranded origin (dso) required for plasmid maintenance in the absence of antibiotic selection, a repF gene encoding for the S. aureus Rep protein and the E. coli R6Kγ replication origin. pKK30 lacks the four predicted USA300-specific open reading frames allowing for use of pKK30 in non-USA300 isolates.
The complete sequence and vector map of pSFRFPS1 are available on the Certificate of Analysis for NR-51165 lot 70010752 and the sequence of pSFRFPS1 is available from GenBank: (MF769789).
Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth containing 10 ?g/mL trimethoprim supplemented with 25% glycerol.
主要内容
此项目的每个订单数量限制为1. 这个项目可以每年订购两次. 此限制的订单将在发货前发送到NIAID批准. NR-51165是&NBSP的甘油库存; 金黄色葡萄球菌,S. aureus),菌株RN4220
含有EQFP650远红荧光蛋白(FRFP)记者质粒PSFRFPS1,
大肠杆菌&nbsp的衍生物; (例如coli)  - 葡萄球菌
班车矢量pkk30. 质粒pkk30是一个
一组穿梭矢量的成员,保持稳定性的稳定性
在没有抗生素选择的情况下,在葡萄球菌中的物种中生物体和.
强大的遗传工具. pkk30包含p sara p1 :: dfra
TrimethoLim抵抗盒,A BLAZ
转录终止子( BLAZ TT),
质粒单链来源( SSO )
质粒复制所需的是质粒维护所需的双链源( DSO )
没有抗生素选择,a repf
S. S的基因编码.金黄色葡萄球菌
Rep蛋白和 e. COLI R6Kγ复制起源. PKK30缺少四个
预测USA300特定的开放阅读框架,可使用PKK30
非美国300孤立.
证书上提供了PSFRFPS1的完整序列和矢量地图
NR-51165 Lot 70010752分析及PSFRFPS1序列
可从Genbank :( mf769789 ). 每个小瓶都包含大约
胰蛋白酶中的0.5毫升细菌培养物
含有10μg/ ml的大豆肉汤,补充25%
甘油.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
品牌标识

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