NR-43495结核分枝杆菌亚种.结核病,H37Rv:pEXCF-3911,转录因子过度表达突变体(突变细菌）
产品图片
货号/SKU
NR-43495
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、3
产品描述信息
NR-43495??Mycobacterium tuberculosis subsp. tuberculosis, H37Rv:pEXCF-3911, Transcription Factor Overexpression Mutant(Mutant Bacteria)|Mycobacterium tuberculosis subsp. tuberculosis|H37Rv:pEXCF-3911, Transcription Factor Overexpression Mutant|-60°C or colder|D ShermanAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis subsp. tuberculosis, Strain H37Rv:pEXCF-3911, Transcription Factor Overexpression Mutant, NR-43495."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Mycobacterium tuberculosis (M. tuberculosis) subsp. tuberculosis, strain H37Rv was transformed with a C-terminally epitope-tagged expression vector containing Rv3911, a possible alternative polymerase sigma factor, SigM.
Fidelity of H37Rv:pEXCF-3911 was confirmed by sequencing prior to transformation into M. tuberculosis. Following transformation, transcription factor expression was assayed by induction with anhydrotetracycline (ATc). The microarray data obtained indicated that ATc led to 3.07 fold over-expression of Rv3911 (see Table 1 on Product Information Sheet for complete microarray data).
Further details relating to applications and the construction of the entire TFOE mutant collection can be found in Nature 499 (2013): 178-183. PubMed: 23823726.
M. tuberculosis, strain H37Rv, was acquired from the Colorado State University TB Vaccine Testing and Research Materials Contract and was sequenced by the Broad Institute (GenBank: CP003248).
Each vial contains approximately 0.25 mL of bacterial culture in Middlebrook 7H9 liquid medium containing 50 ?g/mL hygromycin, 0.2% glycerol, 0.05% Tween80, 0.5% BSA, 0.2% dextrose and 0.085% sodium chloride.
Mycobacterium tuberculosis (M. tuberculosis) subsp. tuberculosis, strain H37Rv was transformed with a C-terminally epitope-tagged expression vector containing Rv3911, a possible alternative polymerase sigma factor, SigM.
Fidelity of H37Rv:pEXCF-3911 was confirmed by sequencing prior to transformation into M. tuberculosis. Following transformation, transcription factor expression was assayed by induction with anhydrotetracycline (ATc). The microarray data obtained indicated that ATc led to 3.07 fold over-expression of Rv3911 (see Table 1 on Product Information Sheet for complete microarray data).
Further details relating to applications and the construction of the entire TFOE mutant collection can be found in Nature 499 (2013): 178-183. PubMed: 23823726.
M. tuberculosis, strain H37Rv, was acquired from the Colorado State University TB Vaccine Testing and Research Materials Contract and was sequenced by the Broad Institute (GenBank: CP003248).
Each vial contains approximately 0.25 mL of bacterial culture in Middlebrook 7H9 liquid medium containing 50 ?g/mL hygromycin, 0.2% glycerol, 0.05% Tween80, 0.5% BSA, 0.2% dextrose and 0.085% sodium chloride.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送到NIAID进行批准. 亚数据.结核菌分，用含有RV3911的C末端表位标记的表达载体转化菌株H37RV，可能的替代聚合酶Sigma因子，Sigm. H37RV的保真度：PEXCF-3911已确认通过在转化到 m之前测序.结核病.在转化后，通过用Anhydrottracycline（ATC）诱导测定转录因子表达.获得的微阵列数据表明，ATC导致RV3911的3.07折叠过表达（参见完整的微阵列数据的产品信息表上的表1）. 关于应用的进一步详情和整个TFOE突变体集合的构建可以在 nature 499（2013）：178-183 PubMed：23823726. m.肺结核，菌株H37RV从科罗拉多州立大学结核病疫苗测试和研究材料合同中获得，并由广泛的研究所（Genbank： CP003248 ）. 每个小瓶在含有50μg/ ml氢霉素的液体培养基中含有大约0.25毫升的细菌培养基，0.2％甘油，0.05％Tween80，0.5 ％BSA，0.2％葡萄糖和0.085％氯化钠.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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