NR-15771来自鼠疫耶尔森氏菌的组分1荚膜抗原(F1),带有N末端组氨酸标签,在大肠杆菌中表达(蛋白质)
产品图片
货号/SKU
NR-15771
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-15771?? Fraction 1 Capsular Antigen (F1) from Yersinia pestis with N-terminal Histidine Tag, Expressed in Escherichia coli(Proteins)|Yersinia pestis|Fraction 1 Capsular Antigen (F1) from Yersinia pestis with N-terminal Histidine Tag, Expressed in Escherichia coli|-80°C or colder|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Fraction 1 Capsular Antigen (F1) from Yersinia pestis with N-terminal Histidine Tag, Expressed in Escherichia coli, NR-15771."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-15771 is a recombinant form of the Yersinia pestis (Y. pestis) fraction 1 capsular antigen (F1). F1 is a plasmid (pFra)-encoded proteinaceous capsule synthesized in large quantities by Y. pestis and reported to confer antiphagocytic properties on the pathogen by interfering with complement-mediated opsonization. The protein is highly immunogenic and has been indirectly associated with eliciting a protective immune response in humans. The recombinant protein lacks the native signal sequence but includes an N-terminal hexa-histidine tag, a thrombin cleavage site, and amino acid residues 22 to 170 of F1 from Y. pestis (GenPept: AAS58714). The protein was expressed in Escherichia coli and purified by nickel affinity chromatography. NR-15771 has a theoretical molecular weight of 17,858 daltons.
Each vial contains approximately 300 ?g of NR-15771 in PBS.
NR-15771 reacts with monoclonal antibody to Y. pestis F1 antigen in ELISA and Western blot assays.
NR-15771 is a recombinant form of the Yersinia pestis (Y. pestis) fraction 1 capsular antigen (F1). F1 is a plasmid (pFra)-encoded proteinaceous capsule synthesized in large quantities by Y. pestis and reported to confer antiphagocytic properties on the pathogen by interfering with complement-mediated opsonization. The protein is highly immunogenic and has been indirectly associated with eliciting a protective immune response in humans. The recombinant protein lacks the native signal sequence but includes an N-terminal hexa-histidine tag, a thrombin cleavage site, and amino acid residues 22 to 170 of F1 from Y. pestis (GenPept: AAS58714). The protein was expressed in Escherichia coli and purified by nickel affinity chromatography. NR-15771 has a theoretical molecular weight of 17,858 daltons.
Each vial contains approximately 300 ?g of NR-15771 in PBS.
NR-15771 reacts with monoclonal antibody to Y. pestis F1 antigen in ELISA and Western blot assays.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
NR-15771是 yersinia Pestis(Y.Pestis)馏分1荚膜抗原(F1)的重组形式. F1是通过 Y以大量合成的质粒(PFRA)的蛋白质胶囊. Pestis 并据报道通过干扰补蛋白介导的调理剂来赋予病原体的抗热性性质.蛋白质是高度免疫原性的,并且与引发人类的保护性免疫应答间接相关.重组蛋白质缺乏天然信号序列,但包括N-末端六氨酸酯标签,凝血酶切割位点和氨基酸残基22-170的F1,从 Y中. pestis (Genpept: AAS58714 ).在大肠杆菌中表达蛋白质,并通过镍亲和层析纯化. NR-15771具有理论分子量为17,858道尔顿.
每个小瓶在PBS中含有大约300μg的NR-15771.
NR-15771与单克隆抗体与 Y反应. ELISA和Western印迹测定中的Pestis F1抗原.
厂牌介绍
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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