产品描述信息
NR-546??Vaccinia virus, (WR) B5R Protein with N-terminal Histidine Tag, Recombinant from Baculovirus (Proteins)|Vaccinia virus|(WR) B5R Protein with N-terminal Histidine Tag, Recombinant from Baculovirus |-60°C or colder|GH Cohen, RJ EisenbergAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus (WR) B5R Protein with N-terminal Histidine Tag, Recombinant from baculovirus, NR-546."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.NR-546 is a recombinant form of the B5R membrane glycoprotein [B5R(275t); residues 20 to 275 comprising the ectodomain, N-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus. The full length B5R protein is 317 residues. NR-546 was produced in Sf9 insect cells using a baculovirus expression system and was purified using ammonium sulfate precipitation and nickel affinity chromatography. NR-546 was demonstrated to be functionally active based on its reactivity with human polyclonal anti-vaccinia virus immune globulin (VIG) and monoclonal antibodies to B5R (BEI Resources NR-422 to NR-424, NR-426 to NR-431, NR-551 to NR-556, and NR-559 to NR-562).Each vial contains approximately 0.5 mg of NR-546 in 50 mM borate buffer (pH 8) containing 100 mM NaCl.