NR-15577猪流感A(H1N1)实时RT-PCR检测(检测/面板）
产品图片
货号/SKU
NR-15577
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-15577?? Swine Influenza A (H1N1) Real-Time RT-PCR Assay(Assays/Panels)|Influenza A virus|Swine Influenza A (H1N1) Real-Time RT-PCR Assay|-20°C or colder|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Swine Influenza A (H1N1) Real-Time RT-PCR Assay, NR-15577."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
The Swine Influenza A (H1N1) Real-Time RT-PCR Assay includes oligonucleotide primers and dual-labeled hydrolysis (TaqMan?) probes to be used in real-time RT-PCR assays for the in vitro qualitative detection and characterization of swine influenza viruses. The TaqMan? probes are labeled with the reporter molecule 6-carboxyfluorescein (6-FAM) and with the quencher, Black Hole Quencher? 1 (BHQ1).
NR-15577 contains the following components: 1) A Universal Influenza A primer and probe set designed to detect type A influenza viruses, 2) A Swine Influenza A primer and probe set designed to detect swine influenza A viruses, 3) A Swine Influenza H1 primer and probe set designed to detect swine H1 influenza A viruses, 4) A Human RNase P Positive Control primer and probe set designed to detect the presence of RNA from the human RNase P gene in human samples, indicating acceptable sample quality, 5) A Swine Influenza A (H1N1) Positive Control containing PBS spiked with beta-propiolactone (BPL)-inactivated swine H1N1 influenza A virus and human epithelial cells, 6) A Novel Influenza A (H1N1) Positive Control containing PBS spiked with BPL-inactivated novel H1N1 influenza A virus and human epithelial cells, and 7) A Human Specimen Extraction Control containing PBS spiked with human epithelial cells.
Primers are provided lyophilized at approximately 20 nmoles per vial. Probes are provided lyophilized at approximately 5 nmoles per vial. Primers and probes should be reconstituted with 500 ?L of nuclease-free water. The Positive Controls and the Extraction Control are provided in 500 ?L volumes.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
The Swine Influenza A (H1N1) Real-Time RT-PCR Assay includes oligonucleotide primers and dual-labeled hydrolysis (TaqMan?) probes to be used in real-time RT-PCR assays for the in vitro qualitative detection and characterization of swine influenza viruses. The TaqMan? probes are labeled with the reporter molecule 6-carboxyfluorescein (6-FAM) and with the quencher, Black Hole Quencher? 1 (BHQ1).
NR-15577 contains the following components: 1) A Universal Influenza A primer and probe set designed to detect type A influenza viruses, 2) A Swine Influenza A primer and probe set designed to detect swine influenza A viruses, 3) A Swine Influenza H1 primer and probe set designed to detect swine H1 influenza A viruses, 4) A Human RNase P Positive Control primer and probe set designed to detect the presence of RNA from the human RNase P gene in human samples, indicating acceptable sample quality, 5) A Swine Influenza A (H1N1) Positive Control containing PBS spiked with beta-propiolactone (BPL)-inactivated swine H1N1 influenza A virus and human epithelial cells, 6) A Novel Influenza A (H1N1) Positive Control containing PBS spiked with BPL-inactivated novel H1N1 influenza A virus and human epithelial cells, and 7) A Human Specimen Extraction Control containing PBS spiked with human epithelial cells.
Primers are provided lyophilized at approximately 20 nmoles per vial. Probes are provided lyophilized at approximately 5 nmoles per vial. Primers and probes should be reconstituted with 500 ?L of nuclease-free water. The Positive Controls and the Extraction Control are provided in 500 ?L volumes.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送至NIAID进行批准.
此项目目前处于我们的生产队列中.请允许充足的时间进行分销批次.
猪流感A（H1N1）实时RT-PCR测定包括寡核苷酸引物和双标记的水解（Taqman?）探针，用于在体外的实时RT-PCR测定中用于实时RT-PCR测定的定性猪流感病毒的检测与表征. Taqman?探针用记者分子6-羧基荧光素（6-Carboxyfluorescin（6-FAM）和Quecher，黑洞Quencher?1（BHQ1）标记.
NR-15577含有以下组分：1）通用流感底漆和探针组，设计用于检测型流感病毒，2）猪流感底漆和探针组，设计用于检测猪流感病毒，3）猪流感H1底漆和探针组设计用于检测猪H1流感的病毒，4）一种人RNase P阳性对照引物和探针组，旨在检测人样品中的人RNase P基因的RNA的存在，表明可接受的样品质量，5）a猪流感A（H1N1）含有PBS的阳性对照掺入β-丙酮（BPL） - 灭活的猪H1N1流感病毒和人性上皮细胞，6）一种新的流感A（H1N1）含有PBS与BPL-灭活新型H1N1的PBS的阳性对照流感病毒和人的上皮细胞，7）含有人的上皮细胞掺入的人类样本提取控制.
引物在每小时约20摩尔下冻干.将探针在每小时约5摩尔的约5摩尔中冻干.引物和探针应用500μL无核酸酶的水重构.阳性对照和提取控制以500μL体积提供.
厂牌介绍
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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