产品描述信息
NR-22132??Vaccinia virus, (WR) B5R Protein with N-Terminal Histidine Tag, Recombinant from Baculovirus(Proteins)|Vaccinia virus|(WR) B5R Protein with N-Terminal Histidine Tag, Recombinant from Baculovirus|-20°C or colder|GH Cohen, RJ EisenbergAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus (WR) B5R Protein with N-Terminal Histidine Tag, Recombinant from Baculovirus, NR-22132."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.A recombinant form of the B5R membrane glycoprotein [B5R (275t)] of the Western Reserve (WR) strain of vaccinia virus containing an N-terminal histidine-tag was produced in Sf9 insect cells using a baculovirus expression system and purified using nickel affinity chromatography. NR-22132 contains residues 20 to 275 of the B5R protein. The full length B5R protein is 317 residues (GenPept: Q01227). NR-22132 was expressed from the same recombinant baculovirus vector as NR-2624, which was produced in cabbage looper (Trichoplusia ni) insect larvae.NR-22132 was demonstrated to be functionally active based on its reactivity with human polyclonal anti-vaccinia virus immune globulin (VIG; BEI Resources NR-650) and mouse monoclonal antibodies to B5R (BEI Resources NR-422 to NR-424, NR-427 to NR-431, NR-552 to NR-554, NR-556 and NR-559 to NR-562).Each vial contains approximately 100 ?g of NR-22132 in approximately 100 ?L of PBS (pH 7.4) with 50% glycerol (v/v).