产品描述信息
NR-50655?? Bacillus anthracis Collagen-like Protein BclA with N-terminal Histidine Tag, Recombinant from Escherichia coli(Proteins)|Bacillus anthracis|Bacillus anthracis Collagen-like Protein BclA with N-terminal Histidine Tag, Recombinant from Escherichia coli|-80°C or colder|BEI ResourcesAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Bacillus anthracis Collagen-like Protein BclA with N-terminal Histidine Tag, Recombinant from Escherichia coli, NR-50655.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.NR-50655
is a recombinant form of the Bacillus
anthracis (B. anthracis) collagen-like
protein (BclA), a major component of the exosporium. The amino acid sequence includes 1) an N-terminal
hexa-histidine tag, 2) a thrombin cleavage site and 3) the complete coding
sequence of BclA from the Sterne strain (GenPept: AAT53453). The recombinant protein was expressed in Escherichia
coli, using an amplified BclA region of the B. anthracis genome (BEI Resources NR-10310), and purified by
nickel affinity chromatography. NR-50655
has a theoretical molecular weight of approximately 39 kilodaltons. The structure of BclA has been solved (PDB: 1WCK;
2R6Q). The predicted amino
acid sequence of NR-50655 is shown on the Product Information Sheet. The collagen-like region of the BclA protein
is known to be highly polymorphic, with a variable number of GXX triplet
motifs, including one to eight copies of the 21 amino acid sequence (GPT)5GDTGTT,
named the BclA repeat. Each
vial contains approximately 80 ?g to 120 ?g of NR-50655 in PBS, pH 7.4. NR-50655 reacts with rabbit polyclonal antibody to B. anthracis BclA (BEI Resources NR-9578) and anti-His monoclonal
antibody as shown by western blot analysis.