NR-50655炭疽杆菌胶原蛋白样蛋白BclA与N末端组氨酸标签,大肠杆菌重组(蛋白质）
产品图片
货号/SKU
NR-50655
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-50655?? Bacillus anthracis Collagen-like Protein BclA with N-terminal Histidine Tag, Recombinant from Escherichia coli(Proteins)|Bacillus anthracis|Bacillus anthracis Collagen-like Protein BclA with N-terminal Histidine Tag, Recombinant from Escherichia coli|-80°C or colder|BEI ResourcesAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Bacillus anthracis Collagen-like Protein BclA with N-terminal Histidine Tag, Recombinant from Escherichia coli, NR-50655.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-50655 is a recombinant form of the Bacillus anthracis (B. anthracis) collagen-like protein (BclA), a major component of the exosporium. The amino acid sequence includes 1) an N-terminal hexa-histidine tag, 2) a thrombin cleavage site and 3) the complete coding sequence of BclA from the Sterne strain (GenPept: AAT53453). The recombinant protein was expressed in Escherichia coli, using an amplified BclA region of the B. anthracis genome (BEI Resources NR-10310), and purified by nickel affinity chromatography. NR-50655 has a theoretical molecular weight of approximately 39 kilodaltons. The structure of BclA has been solved (PDB: 1WCK; 2R6Q). The predicted amino acid sequence of NR-50655 is shown on the Product Information Sheet. The collagen-like region of the BclA protein is known to be highly polymorphic, with a variable number of GXX triplet motifs, including one to eight copies of the 21 amino acid sequence (GPT)5GDTGTT, named the BclA repeat.
Each vial contains approximately 80 ?g to 120 ?g of NR-50655 in PBS, pH 7.4.
NR-50655 reacts with rabbit polyclonal antibody to B. anthracis BclA (BEI Resources NR-9578) and anti-His monoclonal antibody as shown by western blot analysis.
NR-50655 is a recombinant form of the Bacillus anthracis (B. anthracis) collagen-like protein (BclA), a major component of the exosporium. The amino acid sequence includes 1) an N-terminal hexa-histidine tag, 2) a thrombin cleavage site and 3) the complete coding sequence of BclA from the Sterne strain (GenPept: AAT53453). The recombinant protein was expressed in Escherichia coli, using an amplified BclA region of the B. anthracis genome (BEI Resources NR-10310), and purified by nickel affinity chromatography. NR-50655 has a theoretical molecular weight of approximately 39 kilodaltons. The structure of BclA has been solved (PDB: 1WCK; 2R6Q). The predicted amino acid sequence of NR-50655 is shown on the Product Information Sheet. The collagen-like region of the BclA protein is known to be highly polymorphic, with a variable number of GXX triplet motifs, including one to eight copies of the 21 amino acid sequence (GPT)5GDTGTT, named the BclA repeat.
Each vial contains approximately 80 ?g to 120 ?g of NR-50655 in PBS, pH 7.4.
NR-50655 reacts with rabbit polyclonal antibody to B. anthracis BclA (BEI Resources NR-9578) and anti-His monoclonal antibody as shown by western blot analysis.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送到NIAID进行批准. NR-50655
是＆nbsp的重组形式; bacillus
炭疽病＆nbsp; （b.炭疽病）胶原蛋白样
蛋白质（BCLA）是exosporium的主要组分.氨基酸序列包括1）n末端
Hexa-mistiatine标签，2）凝血酶裂解位点和3）完整编码
来自斯诺伯菌株的BCLA序列（Genpept：Aat53453）.重组蛋白在＆中表达; 大肠杆菌
Coli ，使用＆nbsp的扩增BCLA区域; B.炭疽病基因组（Bei资源NR-10310），并纯化
镍亲和层析. NR-50655.
具有大约39千伏多谷的理论分子量. BCLA的结构已经解决（PDB：1WCK;
2R6Q）.＆nbsp;＆nbsp;预测的氨基
NR-50655的酸序列显示在产品信息表上. BCLA蛋白的胶原状区域
已知是高多态性的，具有可变数量的Gxx三联体
图案，包括21个氨基酸序列（GPT） 5 gdtgtt的一到八份拷贝，
命名为BCLA重复.
小瓶在PBS中含有约80μg至120μg的NR-50655，pH 7.4. NR-50655与兔多克隆抗体与 B反应. Anthracis BCLA（Bei Resources NR-9578）和抗他的单克隆
抗体如蛋白质印迹分析所示.
厂牌介绍
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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