产品描述信息
NR-42902??Salmonella enterica subsp. enterica, 14028s (Serovar Typhimurium) Multiple-Gene Deletion Mutant Library, Plate MGD_C03_Cm(Mutant Bacteria)|Salmonella enterica subsp. enterica|14028s (Serovar Typhimurium) Multiple-Gene Deletion Mutant Library, Plate MGD_C03_Cm|-80°C or colder|M McClellandAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Salmonella enterica subsp. enterica, Strain 14028s (Serovar Typhimurium) Multiple-Gene Deletion Mutant Library, Plate MGD_C03_Cm, NR-42902.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. The
Salmonella
enterica (
S. enterica) subsp.
enterica, strain 14028s
(serovar Typhimurium) targeted multiple-gene
deletion (MGD) mutant libraries contain mutants with deletions of contiguous
regions of three or more genes, covering a total of 3,476 genes, between two
collections of mutants differentiated by kanamycin or chloramphenicol
resistance. The
chloramphenicol-resistant MGD mutant collection contains 251 mutants spanning
2,799 genes distributed among three 96-well plates, in which each deleted
region is replaced by a cassette conferring the chloramphenicol resistance
gene. Deletions were confirmed by the depositor. Genes were targeted for deletion by primers designed to preserve the first and last 30 bases of each deleted gene. Gene replacement followed a modified Lambda-Red technique, with an added T7 RNA polymerase promoter positioned in plasmid
pCLF3 to generate a gene-specific transcript from the Salmonella genome directly downstream of each mutant.Note: The strain designation on the plate, strain CDC 6516-60, is incorrect. The correct strain designation is strain 14028s. S. enterica subsp. enterica, strain 14028s was originally known as strain 14028. A variant of the original strain with a rough colony morphology was designated 14028r and the original smooth strain was renamed 14028s. Strain 14028 is a descendent of strain CDC 6516-60, which was isolated from pools of hearts and livers of 4-week-old chickens. The complete genome of S. enterica subsp. enterica, strain 14028s (GenBank:
CP001363.1) and plasmid (GenBank:
CP001362.1) sequences are available.Each inoculated well of the 96-well plate contains approximately 50 ?L of culture in Luria Bertani (LB) broth containing 20 ?g/mL chloramphenicol supplemented with 10% glycerol.Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual mutants provided by the contributor.