NR-42623盘尾丝虫科,用于5SrDNA扩增的丝虫正向S2引物(引物和探针）

NR-42623??Onchocercidae, Filarial Forward S2 Primer for 5S rDNA Amplification(Primers and Probes)|Onchocercidae|Filarial Forward S2 Primer for 5S rDNA Amplification|-20°C or colder|SA WilliamsAcknowledgment for publications should read "The following reagent was provided by the NIH/NIAID Filariasis Research Reagent Resource Center for distribution by BEI Resources, NIAID, NIH: Filarial Forward S2 Primer for 5S rDNA Amplification, NR-42623."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.The filarial S2 forward primer is a 21 nucleotide primer designed to amplify the 5S rDNA when paired with the S16 reverse primer (NR-42624). The S2 and S16 primers are designed to target a highly conserved region of the 5S rDNA and can be used to amplify DNA from the Onchocercidae family including, but not limited to: Brugia, Wuchereria, Onchocerca, Mansonella, Dirofilaria and Ancanthocheilonema genera. The amplified product ranges in size from approximately 400 to 600 base pairs, depending on the species. The sequence of the S2 forward primer is: 5'-GTTAAGCAACGTTGGGCCTGG-3'.For technical questions, please contact genome@smith.edu.Each vial contains approximately 20 ?L of forward primer in nuclease-free TE buffer (1 mM Tris-HCl, 0.1 mM EDTA, pH ~ 8).