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产品图片
货号/SKU
NR-36208
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-36208?? Anthrax Protective Antigen (PA), Recombinant from Bacillus anthracis(Toxins)|Bacillus anthracis|Anthrax Protective Antigen (PA), Recombinant from Bacillus anthracis|2°C to 8°C prior to reconstitution|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Anthrax Protective Antigen (PA), Recombinant from Bacillus anthracis, NR-36208."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Recombinant anthrax protective antigen (PA, 83 kDa) was produced using a plasmid licensed from the NIH. The plasmid was introduced into a non-sporulating avirulent strain of Bacillus anthracis lacking both of the wild type plasmids, pX01 and pX02. Recombinant PA was purified using conventional chromatographic techniques. The resulting purified protein lacks all other anthrax virulence factors.
In vivo, recombinant PA binds to surface receptors on the mammalian cell membrane, and is cleaved by a cellular protease to a 63 kDa protein. When combined with recombinant lethal factor (LF) or edema factor (EF), the cleaved PA binds the toxin enzyme component and mediates its transportation into the cytosol where it exerts its pathogenic effect.
Each vial contains approximately 0.1 mg of recombinant PA. When reconstituted with 0.1 mL of sterile distilled water, the concentration of buffer is 5 mM HEPES (pH 7.5) and 50 mM NaCl. Note: Handle the product gently; DO NOT VORTEX.
Recombinant anthrax protective antigen (PA, 83 kDa) was produced using a plasmid licensed from the NIH. The plasmid was introduced into a non-sporulating avirulent strain of Bacillus anthracis lacking both of the wild type plasmids, pX01 and pX02. Recombinant PA was purified using conventional chromatographic techniques. The resulting purified protein lacks all other anthrax virulence factors.
In vivo, recombinant PA binds to surface receptors on the mammalian cell membrane, and is cleaved by a cellular protease to a 63 kDa protein. When combined with recombinant lethal factor (LF) or edema factor (EF), the cleaved PA binds the toxin enzyme component and mediates its transportation into the cytosol where it exerts its pathogenic effect.
Each vial contains approximately 0.1 mg of recombinant PA. When reconstituted with 0.1 mL of sterile distilled water, the concentration of buffer is 5 mM HEPES (pH 7.5) and 50 mM NaCl. Note: Handle the product gently; DO NOT VORTEX.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送到NIAID进行批准. 使用从NIH授权的质粒产生重组炭疽保护抗原(PA,83kDA).将质粒引入非孢子杆菌的非孢子杆菌菌株中,抑制碱性质粒,PX01和PX02两者.使用常规的色谱技术纯化重组PA.所得纯化的蛋白质缺乏所有其他炭疽毒力因子. 在体内,重组Pa与哺乳动物细胞膜的表面受体结合,并通过细胞蛋白酶切割至63kDa蛋白质.当与重组致命因子(LF)或水肿因子(EF)结合时,切割的PA结合毒素酶组分,并将其运输介导进入细胞溶溶胶,在那里它施加致病作用. 每个小瓶含有约0.1毫克重组Pa.当用0.1ml无菌蒸馏水重构时,缓冲液的浓度为5mm Hepes(pH7.5)和50mM NaCl.注意:轻轻处理产品;不要涡旋.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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