产品描述信息
NR-36210?? Anthrax Edema Factor (EF), Recombinant from Bacillus anthracis(Toxins)|Bacillus anthracis|Anthrax Edema Factor (EF), Recombinant from Bacillus anthracis|2°C to 8°C|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Anthrax Edema Factor (EF), Recombinant from Bacillus anthracis, NR-36210."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval.Recombinant anthrax edema factor (EF, 89 kDa) was produced using a plasmid licensed from the NIH. The plasmid was introduced into a non-sporulating avirulent strain of Bacillus anthracis lacking both of the wild type plasmids, pX01 and pX02. Recombinant EF was purified using conventional chromatographic techniques. The resulting purified protein lacks all other anthrax virulence factors.There is one predicted amino acid difference between NR-36210 and NR-141. NR-141 contains an asparagine, rather than a serine, at amino acid position 415 (amino acid position 444 of the precursor protein for NR-141, amino acid position 447 of precursor EF, GenPept P40136, amino acid position 414 of mature EF, GenPept P40136.There is one expected amino acid difference between NR-36210 and mature EF, GenPept P40136. NR-36210 may have an additional histidine residue at the N-terminus (amino acid position 30 of the precursor protein for NR-36210). This has not yet been confirmed by N-terminal sequencing for NR-36210.Each vial contains 100 ?g of recombinant EF from Bacillus anthracis.