NR-36059血吸虫属,用于在血吸虫中表达的载体pLNHXΔD70,在大肠杆菌中重组(质粒/载体）

NR-36059??Schistosoma spp., Vector pLNHXΔD70 for Expression in Schistosoma, Recombinant in Escherichia coli(Plasmid/Vectors)|Schistosoma spp.|Vector pLNHXΔD70 for Expression in Schistosoma, Recombinant in Escherichia coli|-60°C or colder|P BrindleyAcknowledgment for publications should read "The following reagent was provided by the NIAID Schistosomiasis Resource Center for distribution through BEI Resources, NIAID, NIH: Vector pLNHXΔD70 for Expression in Schistosoma, Recombinant in Escherichia coli, NR-36059."|Quantity limit for this item is 2 per year. Orders over this limit will be sent to NIAID for approval before shipment.Vector pLNHXΔD70 was derived from the Clontech Laboratories vector pLNHX, a vector designed for retroviral gene delivery and expression. Once pLNHX is transfected into a packaging cell line, it can express a transcript containing the viral packaging signal, the neomycin selection marker and a target gene. To produce pLNHXΔD70, pLNHX was digested with XhoI to remove the Drosophila heat shock protein 70 promoter (5,301 bp). pLNHXΔD70 was further modified by insertion of a portion of the cHS4 insulator into the U3 region of the 3'-LTR region to produce pLNHX-cHS4-650 (available from BEI Resources as NR-36060). Both vectors can be used for transduction of Schistosoma.Vector: pLNHXΔD70 (pLNHX sequence is available from Clontech)Selection: Ampicillin (Prokaryotic) and Neomycin (Eukaryotic)Each vial contains approximately 1 mL of Escherichia coli DH5α, transformed with vector pLNHXΔD70, in SOC media (Super Optimal broth with Catabolite repression) supplemented with 15% glycerol.