NR-778牛痘病毒,单克隆抗牛痘(WR)L1R,残基1至185(腹水)(类似于VMC-35)(单克隆抗体）
产品图片
货号/SKU
NR-778
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-778??Vaccinia virus, Monoclonal Anti-Vaccinia (WR) L1R, Residues 1 to 185 (ascites) (Similar to VMC-35) (Monoclonal Antibodies)|Vaccinia virus|Monoclonal Anti-Vaccinia (WR) L1R, Residues 1 to 185 (ascites) (Similar to VMC-35) |-20°C|GH Cohen, RJ EisenbergAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Vaccinia Virus (WR) L1R Protein, Residues 1 to 185 (ascites, Mouse), NR-778."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Mouse monoclonal antibody to a recombinant form of the L1R protein [L1R(185t); residues 1 to 185, C-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus was purified from mouse ascites by protein A affinity chromatography. The mouse B cell hybridoma was generated by the fusion of SP2/0 myeloma cells with immunized BALB/c splenocytes. NR-778 was purified from the same hybridoma as VMC-35. The specificity of VMC-35 was determined by reactivity to L1R(185t) by ELISA and confirmed by Western blot analysis under reducing and non-reducing conditions. VMC-35 does not neutralize the infectivity of the intracellular mature virus (IMV) form of vaccinia virus in BS-C-1 cells using an IMV plaque reduction assay.
The purified monoclonal antibody is provided frozen at 1 mg per vial in phosphate buffered saline (pH 7.3) containing 0.05% sodium azide.
Mouse monoclonal antibody to a recombinant form of the L1R protein [L1R(185t); residues 1 to 185, C-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus was purified from mouse ascites by protein A affinity chromatography. The mouse B cell hybridoma was generated by the fusion of SP2/0 myeloma cells with immunized BALB/c splenocytes. NR-778 was purified from the same hybridoma as VMC-35. The specificity of VMC-35 was determined by reactivity to L1R(185t) by ELISA and confirmed by Western blot analysis under reducing and non-reducing conditions. VMC-35 does not neutralize the infectivity of the intracellular mature virus (IMV) form of vaccinia virus in BS-C-1 cells using an IMV plaque reduction assay.
The purified monoclonal antibody is provided frozen at 1 mg per vial in phosphate buffered saline (pH 7.3) containing 0.05% sodium azide.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在这一限制下订单将在发货前发送到NIAID批准. L1R蛋白的重组形式的小鼠单克隆抗体[L1R（185t）;通过蛋白质A亲和层析从小鼠腹水纯化西方储备（WR）痘苗病毒菌株的残基1至185，C-末端组氨酸标记物.通过具有免疫BALB / C脾细胞的SP2 / 0骨髓瘤细胞的融合产生小鼠B细胞杂交瘤.从与VMC-35相同的杂交瘤纯化NR-778. VMC-35的特异性通过ELISA的反应性决定通过ELISA对L1R（185t）确定，并通过在还原和非还原条件下通过Western印迹分析证实.使用IMV斑块还原测定，VMC-35不中和BS-C-1细胞中痘苗病毒的细胞内成熟病毒（IMV）形式的感染性. 纯化的单克隆抗体在1mg下冷冻含有0.05％叠氮化钠的磷酸盐缓冲盐水（pH7.3）的每个小瓶.
厂牌介绍
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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