产品描述信息
NR-421??Vaccinia virus, Monoclonal Anti-Vaccinia (WR) L1R, Residues 1 to 185 (similar to VMC-6), (produced in vitro) (Monoclonal Antibodies)|Vaccinia virus|Monoclonal Anti-Vaccinia (WR) L1R, Residues 1 to 185 (similar to VMC-6), (produced in vitro) |-20°C or colder|GH Cohen, RJ EisenbergAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Vaccinia Virus (WR) L1R Protein, Residues 1 to 185 (similar to VMC-6), (produced in vitro), NR-421."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.Mouse monoclonal antibody to a recombinant form of the L1R protein [L1R(185t); residues 1 to 185, C-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus was purified from a mouse B cell hybridoma using ammonium sulfate precipitation and size exclusion chromatography. The mouse B cell hybridoma was generated by the fusion of SP2/0 myeloma cells with immunized BALB/c splenocytes. NR-421 was purified from the same hybridoma as VMC-6. The specificity of the antibody was determined by reactivity to L1R(185t) by ELISA and confirmed by Western blot analysis using reducing and non-reducing conditions. The reactivity pattern in ELISA assays using overlapping peptides spanning residues 1 to 185 of L1R indicates that VMC-6 recognizes an epitope within amino acids 118 to 128. VMC-6 neutralizes the infectivity of the intracellular mature virus (IMV) form of vaccinia virus in BS-C-1 cells using an IMV plaque reduction assay.Each vial contains approximately 1.0 mL of purified monoclonal antibody in 50 mM borate buffer (pH 8.0 +/- 0.2) containing 0.1 M sodium chloride and no preservatives.