- 33 次围观
产品图片
货号/SKU
NR-421
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-421??Vaccinia virus, Monoclonal Anti-Vaccinia (WR) L1R, Residues 1 to 185 (similar to VMC-6), (produced in vitro) (Monoclonal Antibodies)|Vaccinia virus|Monoclonal Anti-Vaccinia (WR) L1R, Residues 1 to 185 (similar to VMC-6), (produced in vitro) |-20°C or colder|GH Cohen, RJ EisenbergAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Vaccinia Virus (WR) L1R Protein, Residues 1 to 185 (similar to VMC-6), (produced in vitro), NR-421."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Mouse monoclonal antibody to a recombinant form of the L1R protein [L1R(185t); residues 1 to 185, C-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus was purified from a mouse B cell hybridoma using ammonium sulfate precipitation and size exclusion chromatography. The mouse B cell hybridoma was generated by the fusion of SP2/0 myeloma cells with immunized BALB/c splenocytes. NR-421 was purified from the same hybridoma as VMC-6. The specificity of the antibody was determined by reactivity to L1R(185t) by ELISA and confirmed by Western blot analysis using reducing and non-reducing conditions. The reactivity pattern in ELISA assays using overlapping peptides spanning residues 1 to 185 of L1R indicates that VMC-6 recognizes an epitope within amino acids 118 to 128. VMC-6 neutralizes the infectivity of the intracellular mature virus (IMV) form of vaccinia virus in BS-C-1 cells using an IMV plaque reduction assay.
Each vial contains approximately 1.0 mL of purified monoclonal antibody in 50 mM borate buffer (pH 8.0 +/- 0.2) containing 0.1 M sodium chloride and no preservatives.
Mouse monoclonal antibody to a recombinant form of the L1R protein [L1R(185t); residues 1 to 185, C-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus was purified from a mouse B cell hybridoma using ammonium sulfate precipitation and size exclusion chromatography. The mouse B cell hybridoma was generated by the fusion of SP2/0 myeloma cells with immunized BALB/c splenocytes. NR-421 was purified from the same hybridoma as VMC-6. The specificity of the antibody was determined by reactivity to L1R(185t) by ELISA and confirmed by Western blot analysis using reducing and non-reducing conditions. The reactivity pattern in ELISA assays using overlapping peptides spanning residues 1 to 185 of L1R indicates that VMC-6 recognizes an epitope within amino acids 118 to 128. VMC-6 neutralizes the infectivity of the intracellular mature virus (IMV) form of vaccinia virus in BS-C-1 cells using an IMV plaque reduction assay.
Each vial contains approximately 1.0 mL of purified monoclonal antibody in 50 mM borate buffer (pH 8.0 +/- 0.2) containing 0.1 M sodium chloride and no preservatives.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在这一限制下订单将在发货前发送到NIAID批准. L1R蛋白的重组形式的小鼠单克隆抗体[L1R(185t);使用硫酸铵沉淀和尺寸排阻色谱法从小鼠B细胞杂交瘤中纯化西方储备(WR)疫苗病毒的基韦储备(WR)菌株的残基1至185.通过具有免疫BALB / C脾细胞的SP2 / 0骨髓瘤细胞的融合产生小鼠B细胞杂交瘤.从与VMC-6相同的杂交瘤纯化NR-421.通过ELISA的反应性确定抗体的特异性通过ELISA对L1R(185T)测定,并通过使用还原和非还原条件通过Western印迹分析证实.使用重叠肽的ELISA测定中的反应性模式使用L1R的残基1至185表示,VMC-6识别氨基酸118至128中的表位.VMC-6中和细胞内成熟病毒(IMV)的疫苗病毒形式的感染性使用IMV斑块减少测定的BS-C-1细胞. 每个小瓶在含有0.1M氯化钠的50mM硼酸盐缓冲液(pH 8.0 +/- 0.2)中含有大约1.0ml纯化的单克隆抗体,没有防腐剂.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
品牌标识
可能感兴趣的内容
2021-12-21
2021-12-02
2024-06-22
2022-03-31
2022-04-01
2023-11-14
2021-12-21
2022-04-01
2022-04-01
2021-12-21
2023-10-21
2021-12-21
2022-06-17
2022-04-01
2024-08-16