US/MO/14-18949

NR-52010?? Plasmid pUC19 Containing cDNA from Enterovirus D68, US/MO/14-18949, Infectious Clone pUC-49130(Plasmid/Vectors)|Enterovirus D68|Plasmid pUC19 Containing cDNA from Enterovirus D68, US/MO/14-18949, Infectious Clone pUC-49130| -20°C or colder|R AndinoAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pUC19 Containing cDNA from Enterovirus D68, US/MO/14-18949, Infectious Clone pUC-49130, NR-52010.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Enterovirus species D type 68 (EV-D68), US/MO/14-18949 (GenBank: KM851227) genome was cloned into the Escherichia coli (E. coli) cloning vector pUC19 to generate plasmid pUC-49130. pUC-49130 contains a T7 bacteriophage promoter immediately upstream of the 5’ end of the viral genome. Transfection of rhabdomyosarcoma cells with RNA transcribed in vitro from SalI-linearized plasmid results in production of infectious virus particles. pUC-49130 also contains the beta lactamase gene to provide transformant selection through ampicillin resistance in E. coli. The resulting size of the plasmid is approximately 10,122 base pairs. The complete plasmid sequence is provided on the BEI Resources webpage. The plasmid was produced in E. coli and extracted. NR-52010 has been qualified for use in bacterial transformations. Each vial contains approximately 100 ?L of plasmid DNA in TE buffer (10 mM Tris HCl, 1 mM EDTA, pH 8.0). The vial should be centrifuged prior to opening.

NR-51844??Enterovirus D68, US/MO/14-18949, Mouse-Adapted(Viruses)|Enterovirus D68|US/MO/14-18949, Mouse-Adapted| -60°C or colder |EB TarbetAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Enterovirus D68, US/MO/14-18949, Mouse-Adapted, NR-51844.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Enterovirus D68 (EV-D68), US/MO/14-18949, mouse-adapted was derived from EV-D68, US/MO/14-18949 by thirty sequential passages in AG129 mice before purification. The complete genome of EV-D68, US/MO/14-18949 has been sequenced (GenBank: MH708882).  EV-D68, US/MO/14-18949, mouse-adapted was derived from BEI Resources NR-49130 (EV-D68, US/MO/14-18949, which was isolated from a nasopharyngeal swab taken from a human in Missouri, USA, in August 2014) to improve replication in AG129 mice by thirty sequential passages in mice, and designated EV-D68, US/MO/14-18949, mouse passage 30 (MP30). The mouse-adapted virus was plaque purified in rhabdomyosarcoma (RD) cells and a virus stock was prepared by replication in RD cells. Each vial contains approximately 1 mL of cell lysate and supernatant from RD cells infected with EV-D68, US/MO/14-18949, mouse-adapted. Additional information and tools are available at ViPR (Virus Pathogen Resource).