产品描述信息
NR-43475??Mycobacterium tuberculosis subsp. tuberculosis, H37Rv:pEXCF-3416, Transcription Factor Overexpression Mutant(Mutant Bacteria)|Mycobacterium tuberculosis subsp. tuberculosis|H37Rv:pEXCF-3416, Transcription Factor Overexpression Mutant|-60°C or colder|D ShermanAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis subsp. tuberculosis, Strain H37Rv:pEXCF-3416, Transcription Factor Overexpression Mutant, NR-43475."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.Mycobacterium tuberculosis (M. tuberculosis) subsp. tuberculosis, strain H37Rv was transformed with a C-terminally epitope-tagged expression vector containing Rv3416, a transcriptional regulatory protein, WhiB3.Fidelity of H37Rv:pEXCF-3416 was confirmed by sequencing prior to transformation into M. tuberculosis. Following transformation, transcription factor expression was assayed by induction with anhydrotetracycline (ATc). The microarray data obtained indicated that ATc led to 2.32 fold over-expression of Rv3416 (see Table 1 on Product Information Sheet for complete microarray data).Further details relating to applications and the construction of the entire TFOE mutant collection can be found in
Nature 499 (2013): 178-183. PubMed: 23823726.M. tuberculosis, strain H37Rv, was acquired from the Colorado State University TB Vaccine Testing and Research Materials Contract and was sequenced by the Broad Institute (GenBank:
CP003248).Each vial contains approximately 0.25 mL of bacterial culture in Middlebrook 7H9 liquid medium containing 50 ?g/mL hygromycin, 0.2% glycerol, 0.05% Tween80, 0.5% BSA, 0.2% dextrose and 0.085% sodium chloride.