NR-19616鼠疫耶尔森菌,KIM菌株,Gateway?克隆集,在大肠杆菌中重组,板20(克隆）
产品图片
货号/SKU
NR-19616
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-19616?? Yersinia pestis, Strain KIM, Gateway? Clone Set, Recombinant in Escherichia coli, Plate 20(Clones)|Yersinia pestis|Yersinia pestis, Strain KIM, Gateway? Clone Set, Recombinant in Escherichia coli, Plate 20|-80°C or colder|Pathogen Functional Genomics Resource Center at the J. Craig Venter InstituteAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Yersinia pestis, Strain KIM, Gateway? Clone Set, Recombinant in Escherichia coli, Plate 20, NR-19616."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The Yersinia pestis (Y. pestis), strain KIM, Gateway? clone set consists of 43 plates (plate 2 of this clone set has been discontinued) which contain more than 3600 sequence validated clones from Y. pestis, strain KIM cloned in Escherichia coli (E. coli) DH10B-T1 cells. Each open reading frame was constructed in vector pDONR?221 (Invitrogen?) with an ATG start codon and a TAG stop codon. The sequence was validated by full length sequencing of each clone with greater than 1X coverage and a mutation rate of less than 0.2%.
Information related to the use of Gateway? Clones can be obtained from Invitrogen?. Recombination was facilitated through an attB substrate (attB-PCR product or a linearized attB expression clone) with an attP substrate (pDONR?221) to create an attL-containing entry clone. The entry clone contains recombinational cloning sites, attL1 and attL2 to facilitate gene transfer into a destination vector, M13 forward and reverse priming sites for sequencing and a kanamycin resistance gene for selection. Please refer to the Invitrogen? Gateway? Technology Manual for additional details.
Plate orientation and viability were confirmed for NR-19616.
Each inoculated well of the 96-well plate contains approximately 60 ?L of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) Broth containing 50 ?g/mL kanamycin supplemented with 15% glycerol.
Clone plates are replicated using a BioMek? FX robot. Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources only confirms the clone plate orientation and viability of randomly picked clones. BEI Resources does not confirm or validate individual clone identities provided by the contributor.
The Yersinia pestis (Y. pestis), strain KIM, Gateway? clone set consists of 43 plates (plate 2 of this clone set has been discontinued) which contain more than 3600 sequence validated clones from Y. pestis, strain KIM cloned in Escherichia coli (E. coli) DH10B-T1 cells. Each open reading frame was constructed in vector pDONR?221 (Invitrogen?) with an ATG start codon and a TAG stop codon. The sequence was validated by full length sequencing of each clone with greater than 1X coverage and a mutation rate of less than 0.2%.
Information related to the use of Gateway? Clones can be obtained from Invitrogen?. Recombination was facilitated through an attB substrate (attB-PCR product or a linearized attB expression clone) with an attP substrate (pDONR?221) to create an attL-containing entry clone. The entry clone contains recombinational cloning sites, attL1 and attL2 to facilitate gene transfer into a destination vector, M13 forward and reverse priming sites for sequencing and a kanamycin resistance gene for selection. Please refer to the Invitrogen? Gateway? Technology Manual for additional details.
Plate orientation and viability were confirmed for NR-19616.
Each inoculated well of the 96-well plate contains approximately 60 ?L of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) Broth containing 50 ?g/mL kanamycin supplemented with 15% glycerol.
Clone plates are replicated using a BioMek? FX robot. Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources only confirms the clone plate orientation and viability of randomly picked clones. BEI Resources does not confirm or validate individual clone identities provided by the contributor.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送到NIAID进行批准. yersinia pestiis （ y.Pestis ），菌株金，网关?克隆组由43个板（已停止该克隆集的板2），其包含来自 y的3600多个序列验证的克隆. Pestis ，菌株Kim克隆在大肠杆菌（大肠杆菌）（大肠杆菌） DH10B-T1细胞中.每个开放阅读框架以Vector pdonr?221 （Invitrogen?）用ATG起始密码子和标签停止密码子.通过大于1x覆盖率的每个克隆的全长测序验证序列，突变率小于0.2％. 与使用网关?克隆有关的信息从 Invitrogen?.通过 att b底板（ att b-pCR产物或线性化的 att b表达克隆）促进重组，用 att P衬底（PDONR?221）以产生含有含有ATT L的入口克隆.进入克隆含有重组克隆位点， att l1和 att L2，以促进基因转移到目的载体中，用于测序和卡那霉素抗性基因的目的载体，M13向前和逆向引发位点选择.请参阅Invitrogen?网关?技术手册更多详情. NR-19616确认了板取向和可行性. 96孔板的每个接种孔含有大约60μl的. Coli 培养（菌株DH10B-T1）在含有50μg/ mL卡那霉素的LURIA Bertani（LB）肉汤中，其补充有15％甘油. 克隆板被复制使用生物滤泡? fx机器人. 96孔格式的生产增加了相邻井之间的交叉污染风险.应纯化各个克隆（例如，使用良好的微生物实践的单菌落分离和纯化）并在使用前进行序列验证. Bei Resources仅确认随机挑选的克隆的克隆板取向和可存度. Bei资源不确认或验证贡献者提供的单个克隆身份.
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