NR-19465土拉弗朗西斯菌亚种.土拉菌,菌株SCHUS4,Gateway?克隆集,在大肠杆菌中重组,板8(克隆）
产品图片
货号/SKU
NR-19465
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-19465?? Francisella tularensis subsp. tularensis, Strain SCHU S4, Gateway? Clone Set, Recombinant in Escherichia coli, Plate 8(Clones)|Francisella tularensis subsp. tularensis|Francisella tularensis subsp. tularensis, Strain SCHU S4, Gateway? Clone Set, Recombinant in Escherichia coli, Plate 8|-80°C or colder|Pathogen Functional Genomics Resource Center at the J. Craig Venter InstituteAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Francisella tularensis subsp. tularensis, Strain SCHU S4, Gateway? Clone Set, Recombinant in Escherichia coli, Plate 8, NR-19465."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The Francisella tularensis (F. tularensis) subsp. tularensis, strain SCHU S4, Gateway? clone set consists of 19 plates which contain 1693 sequence validated clones from F. tularensis subsp. tularensis, strain SCHU S4 cloned in Escherichia coli (E. coli) DH10B-T1 cells. Each open reading frame was constructed in vector pDONR?221 (Invitrogen?) with a native start codon and no stop codon. The sequence was validated by full length sequencing of each clone with greater than 1X coverage and a mutation rate of less than 0.2%.
Information related to the use of Gateway? Clones can be obtained from Invitrogen?. Recombination was facilitated through an attB substrate (attB-PCR product or a linearized attB expression clone) with an attP substrate (pDONR?221) to create an attL-containing entry clone. The entry clone contains recombinational cloning sites, attL1 and attL2 to facilitate gene transfer into a destination vector, M13 forward and reverse priming sites for sequencing and a kanamycin resistance gene for selection. Please refer to the Invitrogen? Gateway? Technology Manual for additional details.
Plate viability was confirmed for NR-19465.
Each inoculated well of the 96-well plate contains approximately 60 ?L of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) broth containing 50 ?g/mL kanamycin supplemented with 15% glycerol.
Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual mutants provided by the contributor.
The Francisella tularensis (F. tularensis) subsp. tularensis, strain SCHU S4, Gateway? clone set consists of 19 plates which contain 1693 sequence validated clones from F. tularensis subsp. tularensis, strain SCHU S4 cloned in Escherichia coli (E. coli) DH10B-T1 cells. Each open reading frame was constructed in vector pDONR?221 (Invitrogen?) with a native start codon and no stop codon. The sequence was validated by full length sequencing of each clone with greater than 1X coverage and a mutation rate of less than 0.2%.
Information related to the use of Gateway? Clones can be obtained from Invitrogen?. Recombination was facilitated through an attB substrate (attB-PCR product or a linearized attB expression clone) with an attP substrate (pDONR?221) to create an attL-containing entry clone. The entry clone contains recombinational cloning sites, attL1 and attL2 to facilitate gene transfer into a destination vector, M13 forward and reverse priming sites for sequencing and a kanamycin resistance gene for selection. Please refer to the Invitrogen? Gateway? Technology Manual for additional details.
Plate viability was confirmed for NR-19465.
Each inoculated well of the 96-well plate contains approximately 60 ?L of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) broth containing 50 ?g/mL kanamycin supplemented with 15% glycerol.
Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual mutants provided by the contributor.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将被发送到发货前的批准.
Francisella tularensis（F. tularensis）亚木. Tularensis，菌株Schu S4，网关?克隆组由19个板组成，其中包含来自 f的1693个序列验证的克隆. tularensis 子公司. Tularensis，菌株Schu S4克隆在大肠杆菌（Coli ）DH10B-T1细胞中克隆.每个开放阅读框架以载体 PDONR?221
（ Invitrogen ?）具有本机起始密码子和没有停止密码子.通过每个克隆的全长测序验证序列，其覆盖率大于1x覆盖率，突变率小于0.2％.
与使用网关?克隆有关的信息可以从＆nbsp; Invitrogen ?.重组被促进
通过 att b底板（ att b-pCR产物或线性化 att b表达
用 att p底板（pdonr?221）来产生含有含有的含量
入口克隆.进入克隆包含重组克隆位点， att l1和 att l2
为了促进基因转移到目的载体中，M13前向和逆向引发位点进行测序和用于选择的卡那霉素抗性基因.请参阅Invitrogen? 网关?
技术手册 有关其他详细信息.
对于NR-19465确认了板可行性.
96孔板的每个接种孔含有大约60μl的大肠杆菌培养物（菌株DH10B-T1），含有50μg/ mL Kanamycin的Luria Bertani（LB）肉汤，所述甘油补充有15％甘油.
96孔格式的生产增加了风险
相邻井之间的交叉污染.个别克隆应该是
纯化（例如单落区分离和使用纯化
微生物实践）和使用前序列核实. Bei Resources.
不确认或验证贡献者提供的个体突变体.
厂牌介绍
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