NR-17605牛痘病毒,WesternReserveGenome,VAC(WR)-LoxP-GFP-BAC,在大肠杆菌中重组(细胞库）
产品图片
货号/SKU
NR-17605
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-17605??Vaccinia virus, Western Reserve Genome, VAC(WR)-LoxP-GFP-BAC, Recombinant in Escherichia coli(Cell Banks)|Vaccinia virus|Western Reserve Genome, VAC(WR)-LoxP-GFP-BAC, Recombinant in Escherichia coli|-60°C or colder|B MossAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus Western Reserve Genome, VAC(WR)-LoxP-GFP-BAC, Recombinant in Escherichia coli, NR-17605."|Scientists at for-profit
institutions must contact the National Institute of Allergy and Infectious
Diseases (NIAID), Email: kstemple@niaid.nih.gov before the
reagent can be released. Please specify the name, BEI catalog number and a
brief description of the intended use of the reagent in your email to NIAID.
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The entire vaccinia virus (VACV) Western Reserve (WR) genome (~195 Kb) (NCBI Accession AY243312) with a green fluorescent protein (GFP) sequence and two loxP sites was cloned as a bacterial artificial chromosome (BAC) and grown in Escherichia coli (E. coli) DH10? cells harboring a mini-lambda prophage. The integrated mini-lambda encodes the red recombination system, comprising a 5' to 3' exonuclease (Exo), a single-strand DNA binding protein (Bet), and a nuclease inhibitor (Gam), under the control of the temperature-sensitive λ cI857 repressor. E. coli DH10β/VAC-BAC/λ provides a system to achieve recombination between the VAC-BAC atificial chromosome and electroporated linear DNA with homologous sequences to facilitate production of recombinant VACV expression vectors or mutagenesis of the VACV genome.
Each vial contains approximately 200 ?L of NR-17605 in Luria-Bertani (LB) broth supplemented with 10% glycerol.
The presence of authentic VACV WR and GFP sequences in NR-17605 has been confirmed by PCR amplification and partial nucleotide sequencing. The sequence of the entire VACV WR genome has not been confirmed due to the large size of the plasmid insert. The presence of the integrated mini-lambda prophage also has not been confirmed at BEI Resources.
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The entire vaccinia virus (VACV) Western Reserve (WR) genome (~195 Kb) (NCBI Accession AY243312) with a green fluorescent protein (GFP) sequence and two loxP sites was cloned as a bacterial artificial chromosome (BAC) and grown in Escherichia coli (E. coli) DH10? cells harboring a mini-lambda prophage. The integrated mini-lambda encodes the red recombination system, comprising a 5' to 3' exonuclease (Exo), a single-strand DNA binding protein (Bet), and a nuclease inhibitor (Gam), under the control of the temperature-sensitive λ cI857 repressor. E. coli DH10β/VAC-BAC/λ provides a system to achieve recombination between the VAC-BAC atificial chromosome and electroporated linear DNA with homologous sequences to facilitate production of recombinant VACV expression vectors or mutagenesis of the VACV genome.
Each vial contains approximately 200 ?L of NR-17605 in Luria-Bertani (LB) broth supplemented with 10% glycerol.
The presence of authentic VACV WR and GFP sequences in NR-17605 has been confirmed by PCR amplification and partial nucleotide sequencing. The sequence of the entire VACV WR genome has not been confirmed due to the large size of the plasmid insert. The presence of the integrated mini-lambda prophage also has not been confirmed at BEI Resources.
主要内容
营利科学家
机构必须联系国家过敏和传染性研究所
疾病（NIAID），电子邮件： kstemple@niaid.nih.gov 之前
可以释放试剂.请指定名称，北编目号码和a
简要说明试剂在您的电子邮件中到NIAID. 此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. 整个疫苗病毒（VACV）西方储备（WR）基因组（?195 kB）（NCBI加入AY243312）绿色荧光蛋白（GFP）序列和两种LOXP位点被克隆为细菌人工染色体（BAC），并在大肠杆菌（大肠杆菌）Coli（大肠杆菌）中生长，含有迷你λ前麦芽糖的COLI（大肠杆菌）.集成的Mini-Lambda编码红色重组系统，包含5'至3'外核酸酶（EXO），单链DNA结合蛋白（BET）和核酸酶抑制剂（GAM），下方对温度敏感λCI857阻遏物的控制. e. COLI DH10β/ VAC-BAC /λ提供了一种在VAC-BAC人造染色体和电穿孔线性DNA与同源序列之间进行重组的系统，以促进重组VACV表达载体或VACV基因组的诱变. 每个小瓶含有大约200μl的Luria-Bertani（LB）肉汤中的200μLNR-17605，其补充有10％甘油. 通过PCR扩增和部分核苷酸测序确认了NR-17605中真实VACV WR和GFP序列的存在.由于质粒插入件的大尺寸，尚未确认整个VACV WR基因组的序列.集成的迷你兰达尔的存在也尚未在Bei资源确认.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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