MRA-711A_单克隆抗恶性疟原虫红细胞结合抗原 175 RII

MRA-711A?? Monoclonal Anti-Plasmodium falciparum Erythrocyte Binding Antigen-175 RII, Clone R217 (produced in vitro)(Monoclonal Antibodies)|Plasmodium falciparum|Monoclonal Anti-Plasmodium falciparum Erythrocyte Binding Antigen-175 RII, Clone R217 (produced in vitro)|-20°C or colder|BKL Sim, NIAID/NIHAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Plasmodium falciparum Erythrocyte Binding Antigen-175 RII, Clone R217 (produced in vitro), MRA-711A, contributed by B. Kim Lee Sim and NIAID/NIH.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Antibody Class: IgG1κ Monoclonal antibody prepared against the erythrocyte binding antigen (EBA)-175 of Plasmodium falciparum (P. falciparum) 3D7 was purified from clone R217 hybridoma supernatant by protein G affinity chromatography. EBA-175 is a 175 kDa, P. falciparum parasite ligand at the apical end of the merozoite that binds to its receptor glycophorin A on the surface of erythrocytes in a sialic acid-dependent manner. The monoclonal antibody R217 recognizes a conformational, disulfide-constrained epitope within the F2 domain of region II (RII), under non-reducing conditions only. The B cell hybridoma was generated by the fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from BALB/c mice immunized with purified baculovirus recombinant EBA-175 RII protein (3D7). MRA-711A is functional in western blot, immunofluorescence and ELISA assays. Monoclonal antibody R217 only recognizes late (6-day) hepatocyte stage parasites and mature schizonts. Each vial contains 50 ?L to 100 ?L of purified monoclonal antibody in PBS.