MRA-101 恶性疟原虫

MRA-101??Plasmodium falciparum, Strain 3D7, Gametocyte cDNA Library (Libraries)|Plasmodium falciparum|Plasmodium falciparum, Strain 3D7, Gametocyte cDNA Library|-80°C|R HaywoodAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmodium falciparum, Strain 3D7 Gametocyte cDNA Library, MRA-101, contributed by R. Haywood."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material. This is a gametocyte specific library from P. falciparum, strain 3D7. cDNA was prepared from stage III - V gametocytes. Constructed using a Stratagene Uni-ZAP XR vector kit, cDNA synthesis kit, and Gigapack III gold packaging extract (#200450). To date, two genes have been cloned from this library: phosphoenolpyruvate carboxykinase and CTRP-like gene (unpublished). cDNA size fractionation column fractions 5 and 6 were pooled and used for library construction. These fractions correspond to sizes >2 kb. No attempts have been made to isolate any asexual stage genes from the library. Recent titration (19JUL1999): 50 ?L of a 1,000 dilution of the library results in ?,000 pfu. cDNAs were synthesized from stage III - V gametocyte poly(A)+ RNA by oligo d(T) priming, size-selected (>2 Kbp) and directionally cloned into the EcoRI (5' end) to XhoI (3' end) sites of the Uni-ZAP XR lambda vector (Stratagene). Corresponding phagemid vector: pBluescript SK plus; Site_1: EcoRI; Site_2: XhoI; Lab host: E. coli XL1-Blue.