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产品图片
货号/SKU
MRA-336
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
MRA-336?? Anopheles gambiae Complex, PCR Identification Primer Kit (Primers and Probes)|Anopheles gambiae|Anopheles gambiae Complex, PCR Identification Primer Kit |-20°C (-80°C long term storage)|MQ BenedictAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Anopheles gambiae Complex, PCR Identification Primer Kit, MRA-336, contributed by Mark Q. Benedict."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material. Please contact BEI Resources for directions for use.
MRA-336 contains a set of 9 cryotubes containing dried oligonucleotides (shown below) for identification of members of the Anopheles gambiae complex by PCR, after preliminary morphological identification as members of the species complex. Two tubes of UN and AR are supplied; all other primers are unique. The product sizes predicted are those obtained by amplification of the ribosomal DNA (rDNA) intergenic spacer according to the references below. Products are typically resolved by agarose gel electrophoresis and compared to molecular weight standards. Use of positive and negative controls is strongly encouraged.
Primer name/species/fragment length/?g per tube/sequence
UN/all/not applicable/12.5/GTGTGCCCCTTCCTCGATGT
GA/gambiae/390 bp/6.25/CTGGTTTGGTCGGCACGTTT
AR/arabiensis/315 bp/18.75/AAGTGTCCTTCTCCATCC TA
ME/melas, merus/464 bp, 466 bp/12.5/TGACCAACCCACTCCCTTGA
BW/bwambe/152 bp/6.25/GACCAAGATGGTTAGTTAACA
QD/quadriannulatus/153 bp/25/CAGACCAAGATGGTTAGTAT
QDA/alternate quadriannulatus/415 bp/25/CATAATGAGTGCACAGCATA
Note: Use of the QDA primer is recommended when samples are collected from locations in which A. merus and A. quadriannulatus may be sympatric.
Scott, J. A., W. G. Brogdon and F. H. Collins. "Identification of Single Specimens of the Anopheles gambiae Complex by the Polymerase Chain Reaction." Am. J. Trop. Med. Hyg. 49 (1993): 520-529. PubMed: 8214283.
Cornel, A. J. and F. H. Collins. "PCR of the Ribosomal DNA Intergenic Spacer Regions as a Method for Identifying Mosquitoes in the Anopheles gambiae Complex." Methods Mol. Biol. 50 (1996): 321-332. PubMed: 8751368.
Collins, F. H., et al. "A Ribosomal RNA Gene Probe Differentiates Member Species of the Anopheles gambiae Complex." Am. J. Trop. Med. Hyg. 37 (1987): 37-41. PubMed: 2886070.
Rafferty, C. S., et al. "Polymerase Chain Reaction-Based Identification and Genotyping of Anopheles Mosquitoes with a 96-Pin Bacterial Replicator." Am. J. Trop. Med. Hyg. 66 (2002): 234-237. PubMed: 12139213.
This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material. Please contact BEI Resources for directions for use.
MRA-336 contains a set of 9 cryotubes containing dried oligonucleotides (shown below) for identification of members of the Anopheles gambiae complex by PCR, after preliminary morphological identification as members of the species complex. Two tubes of UN and AR are supplied; all other primers are unique. The product sizes predicted are those obtained by amplification of the ribosomal DNA (rDNA) intergenic spacer according to the references below. Products are typically resolved by agarose gel electrophoresis and compared to molecular weight standards. Use of positive and negative controls is strongly encouraged.
Primer name/species/fragment length/?g per tube/sequence
UN/all/not applicable/12.5/GTGTGCCCCTTCCTCGATGT
GA/gambiae/390 bp/6.25/CTGGTTTGGTCGGCACGTTT
AR/arabiensis/315 bp/18.75/AAGTGTCCTTCTCCATCC TA
ME/melas, merus/464 bp, 466 bp/12.5/TGACCAACCCACTCCCTTGA
BW/bwambe/152 bp/6.25/GACCAAGATGGTTAGTTAACA
QD/quadriannulatus/153 bp/25/CAGACCAAGATGGTTAGTAT
QDA/alternate quadriannulatus/415 bp/25/CATAATGAGTGCACAGCATA
Note: Use of the QDA primer is recommended when samples are collected from locations in which A. merus and A. quadriannulatus may be sympatric.
Scott, J. A., W. G. Brogdon and F. H. Collins. "Identification of Single Specimens of the Anopheles gambiae Complex by the Polymerase Chain Reaction." Am. J. Trop. Med. Hyg. 49 (1993): 520-529. PubMed: 8214283.
Cornel, A. J. and F. H. Collins. "PCR of the Ribosomal DNA Intergenic Spacer Regions as a Method for Identifying Mosquitoes in the Anopheles gambiae Complex." Methods Mol. Biol. 50 (1996): 321-332. PubMed: 8751368.
Collins, F. H., et al. "A Ribosomal RNA Gene Probe Differentiates Member Species of the Anopheles gambiae Complex." Am. J. Trop. Med. Hyg. 37 (1987): 37-41. PubMed: 2886070.
Rafferty, C. S., et al. "Polymerase Chain Reaction-Based Identification and Genotyping of Anopheles Mosquitoes with a 96-Pin Bacterial Replicator." Am. J. Trop. Med. Hyg. 66 (2002): 234-237. PubMed: 12139213.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在这一限制下订单将在发货前发送到北欧以批准. 该试剂由贡献者认证.北极资源没有确认或验证这种材料.请联系Bei Resources for Directions使用. MRA-336包含一组9个包含干寡核苷酸(如下所示的Croudotubes),用于鉴定 anophelesGambiae 的成员PCR复合物,初步形态鉴定作为物种复合物的成员.提供联合国和Ar的两根管;所有其他引物都是独一无二的.预测的产品尺寸是通过根据以下参考的参考的参考通过扩增核糖体DNA(RDNA)代骨间隔物而获得的产品尺寸.产品通常通过琼脂糖凝胶电泳来解决并与分子量标准进行比较.强烈鼓励使用正面和阴性对照. 每管/序列/μg gambiae / 390 bp / 6.25 / ctggtttggtcgcgcacgttttt ar / rabiensis / 315 bp / 18.75 / aagtgtccttctctctcc ta Me / Melas,Merus / 464 bp,466 bp / 12.5 / tgaccaaccccactcccttga bw / bwambe / 152 bp / 6.25 / gaccaagatggttagttaaca qd / quadriannulatus / 153 bp / 25 / cagaccaagatggttagtagtaT QDA /备用 Quadriannulatus / 415 BP / 25 / Cataatgagtgcacagcata 注意:当从 a的位置收集样品时,建议使用QDA引物. merus 和 a. Quadriannulatus 可能是合作伙伴. 斯科特,J.A.,W. G. G. Brogdon和F. H. Collins. “通过聚合酶链反应鉴定 anophelesGambiae的单一标本复合物.”你. J. Trop.医学. HYG. 49(1993):520-529. PubMed:8214283. Cornel,A. J.和F. H. Collins. “核糖体DNA代骨间隔区的PCR作为识别蚊子中的方法,anophelesGambiae 复合物.” 方法摩尔. BIOL. 50(1996):321-332. PubMed:8751368. Collins,F. H.等人. “核糖体RNA基因探针区分 anophelesGambiae的成员物种复合物.”你. J. Trop.医学. HYG. 37(1987):37-41. PubMed:2886070. Rafferty,C. S.等人. “基于聚合酶链反应的鉴定和基因分型肌肉肌肉,具有96针细菌复合器的蚊子.”你. J. Trop.医学. HYG. 66(2002):234-237. PubMed:12139213.
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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