产品描述信息
NR-49806??Escherichia coli, K-12, Strain IM08B(Bacteria)|Escherichia coli|K-12, Strain IM08B|-60°C or colder|I Monk, T Stinear, T FosterAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Escherichia coli K-12, Strain IM08B, NR-49806."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Escherichia coli (E. coli) K-12, strain IM08B is an insertion mutant produced in E. coli K-12, strain DC10B via recombination-mediated genetic engineering (recombineering), and contains the hsdM (methylase) and hsdS (specificity) genes from Staphylococcus aureus (S. aureus), strain NRS384 clonal complex 8 (CC8).
E. coli K-12, strain DC10B is a universal host for constructing plasmids for introduction into staphylococci and was deposited as Δdcm. The lack of methylation allows plasmid DNA to bypass a conserved type IV restriction-modification (RM) barrier in staphylococci which has been identified as a major barrier to transformation with foreign DNA. E. coli K-12, strain IM08B was deposited as resistant to streptomycin.
Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth containing 25 ?g per mL streptomycin supplemented with 10% glycerol.