分离株 hCoV-19/USA/WI-IRI-001/2021(B.1.630 谱系)(病毒），NR-1_Vaccinia virus

NR-55697??SARS-Related Coronavirus 2, Isolate hCoV-19/USA/WI-IRI-001/2021 (Lineage B.1.630)(Viruses)|SARS-Related Coronavirus 2|Isolate hCoV-19/USA/WI-IRI-001/2021 (Lineage B.1.630)|-60°C or colder|P Halfmann, Y KawaokaAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-Related Coronavirus 2, Isolate hCoV-19/USA/WI-IRI-001/2021 (Lineage B.1.630), NR-55697, contributed by Peter Halfmann and Yoshihiro Kawaoka.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate hCoV-19/USA/WI-IRI-001/2021 was isolated from a nasal swab from a human in Madison, Wisconsin, USA, in July 2021. Note: Genome sequence information is provided on the Certificate of Analysis and includes an analysis of all sequence variations observed for each lot. Note: The reference sequence used in this analysis, EPI_ISL_3190270, differs from EPI_ISL_5335935, the passage 1 sequence submitted for this virus. Due to quality issues in EPI_ISL_5335935, this sequence was not ideal for comparing sequence of NR-55697. Selection of EPI_ISL_3190270 as the reference sequence was accomplished by mapping the in-house sequence reads to the Wuhan-Hu-1 sequence, generating a consensus sequence, and submitting that sequence to the GISAID Audacity Instant tool to find the most closely related sequence. While EPI_ISL_3190270 and EPI_ISL_5335935 are different, both belong to the Pango Lineage B.1.630, and all of the amino acid mutations characteristic of this lineage are present in the NR-55697 sequence. Note: Due to the dynamic nature of lineage determination using PANGO, it is recommended to confirm provided GISAID information prior to initiation of work. Under the nomenclature system introduced by GISAID (Global Initiative on Sharing All Influenza Data), SARS-CoV-2, isolate hCoV-19/USA/WI-IRI-001/2021 is assigned lineage B.1.630 (Pango v.3.1.16 2021-11-25) and GISAID clade GH using Phylogenetic Assignment of Named Global Outbreak lineages (PANGO) tool. The complete genome of the clinical isolate of SARS-CoV-2, hCoV-19/USA/WI-IRI-001/2021 has been sequenced (GISAID: EPI_ISL_5335935). The following mutations are present in the clinical isolate: Spike A222V, Spike A243del, Spike C1253stop, Spike D614G, Spike D950N, Spike E484Q, Spike H655Y, Spike L244del, Spike N185K, Spike P9L, Spike T478R, N (Nucleocapsid) P80R, N S412N, N T205I, NS3 A39del, NS3 F43del, NS3 G44del, NS3 I37del, NS3 L41del, NS3 L46del, NS3 P36del, NS3 P42del, NS3 Q38del, NS3 Q57H, NS3 S40del, NS3 W45del, NS7b L14S, NSP1 (Non-structural protein 1) L122F, NSP2 (Non-structural protein 2) T85I, NSP2 T153A, NSP3 (Non-structural protein 3) K977Q, NSP3 Q774R, NSP3 R748S, NSP4 (Non-structural protein 4) A446V, NSP5 (Non-structural protein 5) V104I, NSP6 (Non-structural protein 6) F108del, NSP6 G107del, NSP6 S106del, NSP12 (Non-structural protein 12) P323L. There is a gap of 51 nucleotides when compared to the reference sequence. It was designated an unnamed Variant Under Monitoring (VUM) by the World Health Organization (WHO). Each vial contains approximately 0.1 mL of spin-clarified cell lysate and supernatant from Homo sapiens lung adenocarcinoma epithelial cells (Calu-3) infected with SARS-CoV-2, isolate hCoV-19/USA/WI-IRI-001/2021. Additional information and tools are available at ViPR (Virus Pathogen Resource).