巨噬细胞系

NR-19976??Mus musculus, Macrophage Cell Line Derived from TLR2/TLR9 Double Knockout Mice(Cell Banks)|Mus musculus|Macrophage Cell Line Derived from TLR2/TLR9 Double Knockout Mice|-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer|D GolenbockAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Macrophage Cell Line Derived from TLR2/TLR9 Double Knockout Mice, NR-19976."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.The murine macrophage cell line, NR-19976, was derived using primary bone marrow cells from TLR2 (toll-like receptor 2)/TLR9 (toll-like receptor 9) double knockout mice. The primary bone marrow cells were immortalized by infection with the ecotropic transforming replication-deficient retrovirus J2 using techniques described in the literature. Characterization based on immunofluorescence, stimulation assays and flow cytometry demonstrated that the immortalized cell line retains its macrophage-specific morphological, functional and surface expression properties.Each vial contains approximately 1 mL of cell culture suspension frozen in cell growth medium (90%) and DMSO (10%) cryopreservative. Sufficient cells are provided to initiate at least one new culture.

NR-19975??Mus musculus, Macrophage Cell Line Derived from TLR2/TLR4 Double Knockout Mice(Cell Banks)|Mus musculus|Macrophage Cell Line Derived from TLR2/TLR4 Double Knockout Mice|-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer|D GolenbockAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Macrophage Cell Line Derived from TLR2/TLR4 Double Knockout Mice, NR-19975."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. The murine macrophage cell line, NR-19975, was derived using primary bone marrow cells from TLR2 (toll-like receptor 2)/TLR4 (toll-like receptor 4) double knockout mice. The primary bone marrow cells were immortalized by infection with the ecotropic transforming replication-deficient retrovirus J2 using techniques described in the literature. Characterization based on immunofluorescence, stimulation assays and flow cytometry demonstrated that the immortalized cell line retains its macrophage-specific morphological, functional and surface expression properties. Each vial contains approximately 1 mL of cell culture suspension frozen in cell growth medium (90%) and DMSO (10%) cryopreservative. Sufficient cells are provided to initiate at least one new culture.