产品描述信息
NR-15260??Homo sapiens, Monoclonal Anti-Human Interferon Beta Protein, Clone A7 (produced in vitro)(Monoclonal Antibodies)|Homo sapiens|Monoclonal Anti-Human Interferon Beta Protein, Clone A7 (produced in vitro)|-20°C or colder|SE GrossbergAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Human Interferon Beta Protein, Clone A7 (produced in vitro), NR-15260."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Antibody Class: IgG2aκ
Mouse monoclonal antibody prepared against a recombinant form of the human interferon beta (IFN-β) protein was purified from clone A7 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63Ag8.653 myeloma cells with splenocytes from a BALB/c x DBA F1 mouse immunized repeatedly with recombinant human IFN-β protein in adjuvant. The clone A7 antibody is specific for human IFN-β and does not cross-react with IFN-α or IFN-γ.
NR-15260 recognizes recombinant human IFN-β in western blot assays. The clone A7 monoclonal antibody is also reported to function in ELISA, and to neutralize the anti-viral and anti-proliferative effects of unglycosylated recombinant IFN-β, but not of glycosylated natural IFN-β. The clone A7 antibody has been shown to recognize an epitope in the C1 helix of the IFN-β molecule, a site distant from the interferon α/β receptor subunit 2 (INFAR-2) binding residues.
Each vial of NR-15260 contains approximately 100 ?L of purified monoclonal antibody in PBS.