NR-42021单克隆抗甲型流感病毒H1血凝素(HA),A/California/04/2009(H1N1)pdm09,克隆4F8(体外生产)(单克隆抗体)
产品图片
货号/SKU
NR-42021
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-42021?? Monoclonal Anti-Influenza A Virus H1 Hemagglutinin (HA), A/California/04/2009 (H1N1)pdm09, Clone 4F8 (produced in vitro)(Monoclonal Antibodies)|Influenza A virus|Monoclonal Anti-Influenza A Virus H1 Hemagglutinin (HA), A/California/04/2009 (H1N1)pdm09, Clone 4F8 (produced in vitro)|-20°C or colder|JP WeirAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Influenza A Virus H1 Hemagglutinin (HA), A/California/04/2009 (H1N1)pdm09, Clone 4F8 (produced in vitro), NR-42021.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Antibody Class: IgG2aκ
Mouse monoclonal antibody prepared against the H1 hemagglutinin (HA) protein of the A/California/04/2009 (H1N1)pdm09 strain of influenza A virus was purified from clone 4F8 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of mouse myeloma cells with splenocytes from BALB/c mice immunized and boosted with virus-like particles containing only the influenza virus A/California/04/2009 (H1N1)pdm09 HA.
HA is an antigenic glycoprotein found on the envelope of the influenza A virus. This protein binds to cellular receptors on the target cell and allows the influenza A virus to enter via endocytosis and membrane fusion. HA is an important target for drug and vaccine development.
NR-42021 recognizes influenza A virus A/California/04/2009 (H1N1)pdm09-infected cells in indirect immunofluorescence assays, and neutralizes A/California/04/2009 (H1N1)pdm09 in vitro. Clone 4F8 antibody is also reported to function in ELISA, hemagglutination inhibition, and western blot assays.
Each vial of NR-42021 contains approximately 100 ?L of purified monoclonal antibody in PBS.
Antibody Class: IgG2aκ
Mouse monoclonal antibody prepared against the H1 hemagglutinin (HA) protein of the A/California/04/2009 (H1N1)pdm09 strain of influenza A virus was purified from clone 4F8 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of mouse myeloma cells with splenocytes from BALB/c mice immunized and boosted with virus-like particles containing only the influenza virus A/California/04/2009 (H1N1)pdm09 HA.
HA is an antigenic glycoprotein found on the envelope of the influenza A virus. This protein binds to cellular receptors on the target cell and allows the influenza A virus to enter via endocytosis and membrane fusion. HA is an important target for drug and vaccine development.
NR-42021 recognizes influenza A virus A/California/04/2009 (H1N1)pdm09-infected cells in indirect immunofluorescence assays, and neutralizes A/California/04/2009 (H1N1)pdm09 in vitro. Clone 4F8 antibody is also reported to function in ELISA, hemagglutination inhibition, and western blot assays.
Each vial of NR-42021 contains approximately 100 ?L of purified monoclonal antibody in PBS.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
抗体类:IgG2aκ
通过蛋白G亲和色谱法从克隆4F8杂交瘤上清液中纯化针对A / CALIFORNIA / 04/2009(H1N1)PDM09菌株的H1血凝素(HA)蛋白的小鼠单克隆抗体. B细胞杂交瘤由小鼠骨髓瘤细胞与来自Balb / C小鼠的脾细胞的融合产生,并用仅含有流感病毒A / CALIFORNIA / 04/2009(H1N1)PDM09 HA的病毒样颗粒升压.
HA是在流感的血蛋白上发现的抗原糖蛋白.该蛋白质与靶细胞上的细胞受体结合,并允许流感病毒通过内吞作用和膜融合进入. HA是药物和疫苗开发的重要目标.
NR-42021识别流感A / CALIFORNIA / 04/2009(H1N1)PDM09感染细胞在间接免疫荧光测定中,中和A / CALIFORNIA / 04/2009(H1N1)PDM09 体外).还报道克隆4F8抗体在ELISA,血凝抑制和Western印迹测定中起作用.
每个小瓶NR-42021在PBS中含有约100μl纯化的单克隆抗体.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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