克隆 11E10(体外生产)(单克隆抗体），NR-1_Vaccinia virus

NR-45117??Escherichia coli, Monoclonal Anti–Shiga–Like Toxin 2 Subunit A, Clone 11E10 (produced in vitro)(Monoclonal Antibodies)|Escherichia coli|Monoclonal Anti–Shiga–Like Toxin 2 Subunit A, Clone 11E10 (produced in vitro)|-20°C or colder|AD O'Brien, JF SinclairAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Shiga-Like Toxin 2 Subunit A, Clone 11E10 (produced in vitro), NR-45117."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Antibody Class: IgG1κ Mouse monoclonal antibody prepared against the A subunit of Shiga-like toxin 2 from Escherichia coli (E. coli) was purified from clone 11E10 (ATCC ^? CRL-1907?) hybridoma supernatant by protein G affinity chromatography. The hybridoma cell line was generated by the fusion of SP2/0 myeloma cells with immunized mouse splenocytes. The term Shiga toxin (Stx) refers to two families of related toxins: Shiga toxin/Shiga-like toxin 1 and Shiga-like toxin 2. Shiga toxin is produced by Shigella dysenteriae, while Shiga-like toxin 1 and Shiga-like toxin 2 are both produced by enterohemorrhagic strains of E. coli. Both Shiga and Shiga-like toxins are multimeric molecules that are comprised of two polypeptide subunits, A and B. The B subunit is a pentamer that binds to glycolipids on host cell membranes and the entire Stx molecule can then enter the cell via endocytosis. Once inside the cell, the Stx A subunit undergoes proteolytic cleavage and the reduction of an internal disulfide bond to generate Stx A₁ and Stx A₂. Stx A₁ is an N-glycosidase that catalytically inactivates the 28S ribosomal RNA subunit to inhibit protein synthesis. The nucleotide sequence of the gene for the Shiga-like toxin 2 A subunit from E. coli has been reported (GenBank: AB035143). NR-45117 is being released without confirmation of functional activity. The monoclonal antibody produced by hybridoma clone 11E10 has been reported to react with recombinant E. coli Shiga-like toxin 2 subunit A by ELISA and western blot. Each vial of NR-45117 contains approximately 100 ?L of purified monoclonal antibody in PBS, pH 7.4.

NR-36168?? Monoclonal Anti-Hantaan Virus Gc Glycoprotein, Clone 11E10 (produced in vitro)(Monoclonal Antibodies)|Hantaan virus|Monoclonal Anti-Hantaan Virus Gc Glycoprotein, Clone 11E10 (produced in vitro)|-20°C or colder|CS SchmaljohnAcknowledgment for publications should read "The following reagent was obtained from the Joel M. Dalrymple - Clarence J. Peters USAMRIID Antibody Collection through BEI Resources, NIAID, NIH: Monoclonal Anti-Hantaan Virus Gc Glycoprotein, Clone 11E10 (produced in vitro), NR-36168."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.Antibody Class: IgG1κMouse monoclonal antibody prepared against the Hantaan virus Gc (formerly G2) glycoprotein was purified from clone 11E10 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from mice immunized with protein A sepharose-bound Hantaan virus glycoprotein-antibody complexes as described by Arikawa et al.This reagent is part of the Joel M. Dalrymple - Clarence J. Peters USAMRIID Antibody Collection.Each vial of NR-36168 contains approximately 100 ?L of purified monoclonal antibody in PBS.NR-36168 is reactive in indirect immunofluorescence assays using Vero E6 cells infected with Hantaan virus, and neutralizes Hantaan virus in plaque reduction neutralization tests. The antibody is also reported to function in ELISA, hemagglutination inhibition and immunoprecipitation assays, and to cross-react on Prospect Hill virus, Puumala virus, and Sapporo rat virus.