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产品图片
货号/SKU
NR-45117
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-45117??Escherichia coli, Monoclonal Anti–Shiga–Like Toxin 2 Subunit A, Clone 11E10 (produced in vitro)(Monoclonal Antibodies)|Escherichia coli|Monoclonal Anti–Shiga–Like Toxin 2 Subunit A, Clone 11E10 (produced in vitro)|-20°C or colder|AD O'Brien, JF SinclairAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Shiga-Like Toxin 2 Subunit A, Clone 11E10 (produced in vitro), NR-45117."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Antibody Class: IgG1κ
Mouse monoclonal antibody prepared against the A subunit of Shiga-like toxin 2 from Escherichia coli (E. coli) was purified from clone 11E10 (ATCC ? CRL-1907?) hybridoma supernatant by protein G affinity chromatography. The hybridoma cell line was generated by the fusion of SP2/0 myeloma cells with immunized mouse splenocytes.
The term Shiga toxin (Stx) refers to two families of related toxins: Shiga toxin/Shiga-like toxin 1 and Shiga-like toxin 2. Shiga toxin is produced by Shigella dysenteriae, while Shiga-like toxin 1 and Shiga-like toxin 2 are both produced by enterohemorrhagic strains of E. coli. Both Shiga and Shiga-like toxins are multimeric molecules that are comprised of two polypeptide subunits, A and B. The B subunit is a pentamer that binds to glycolipids on host cell membranes and the entire Stx molecule can then enter the cell via endocytosis. Once inside the cell, the Stx A subunit undergoes proteolytic cleavage and the reduction of an internal disulfide bond to generate Stx A1 and Stx A2. Stx A1 is an N-glycosidase that catalytically inactivates the 28S ribosomal RNA subunit to inhibit protein synthesis. The nucleotide sequence of the gene for the Shiga-like toxin 2 A subunit from E. coli has been reported (GenBank: AB035143).
NR-45117 is being released without confirmation of functional activity. The monoclonal antibody produced by hybridoma clone 11E10 has been reported to react with recombinant E. coli Shiga-like toxin 2 subunit A by ELISA and western blot.
Each vial of NR-45117 contains approximately 100 ?L of purified monoclonal antibody in PBS, pH 7.4.
Antibody Class: IgG1κ
Mouse monoclonal antibody prepared against the A subunit of Shiga-like toxin 2 from Escherichia coli (E. coli) was purified from clone 11E10 (ATCC ? CRL-1907?) hybridoma supernatant by protein G affinity chromatography. The hybridoma cell line was generated by the fusion of SP2/0 myeloma cells with immunized mouse splenocytes.
The term Shiga toxin (Stx) refers to two families of related toxins: Shiga toxin/Shiga-like toxin 1 and Shiga-like toxin 2. Shiga toxin is produced by Shigella dysenteriae, while Shiga-like toxin 1 and Shiga-like toxin 2 are both produced by enterohemorrhagic strains of E. coli. Both Shiga and Shiga-like toxins are multimeric molecules that are comprised of two polypeptide subunits, A and B. The B subunit is a pentamer that binds to glycolipids on host cell membranes and the entire Stx molecule can then enter the cell via endocytosis. Once inside the cell, the Stx A subunit undergoes proteolytic cleavage and the reduction of an internal disulfide bond to generate Stx A1 and Stx A2. Stx A1 is an N-glycosidase that catalytically inactivates the 28S ribosomal RNA subunit to inhibit protein synthesis. The nucleotide sequence of the gene for the Shiga-like toxin 2 A subunit from E. coli has been reported (GenBank: AB035143).
NR-45117 is being released without confirmation of functional activity. The monoclonal antibody produced by hybridoma clone 11E10 has been reported to react with recombinant E. coli Shiga-like toxin 2 subunit A by ELISA and western blot.
Each vial of NR-45117 contains approximately 100 ?L of purified monoclonal antibody in PBS, pH 7.4.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
抗体类：IgG1κ
小鼠单克隆抗体从大肠杆菌中抵抗诸如Shiga样毒素2的亚基
通过蛋白G亲和色谱法从克隆11e10（ATCC ? CRL-1907?）杂交瘤上清液中纯化Coli（大肠杆菌）.杂交瘤细胞系由SP2 / 0骨髓瘤细胞的融合产生，具有免疫小鼠脾细胞.
术语毒药毒素（STX）是指两个相关毒素的两个家庭：Shiga毒素/滋阴样毒素1和滋生毒素2.滋阴样毒素2.滋阴类毒素由志贺菌诱因（Shigella dysenteriae）产生，而Shiga样毒素1和Shiga样毒素2均由肠杆菌菌株的 e产生. Coli . Shiga和Shiga样毒素都是由两种多肽亚基，A和B组成的多聚体分子.b亚基是与宿主细胞膜上与糖脂结合的五聚体，并且整个STX分子可以通过内吞作用进入细胞.一旦在细胞内，STX A亚基经历蛋白水解裂解和内部二硫键的还原以产生STX A 1 和STX A 2 . STX A 1 是一种催化灭活28s核糖体RNA亚基以抑制蛋白质合成的 n 糖苷酶. Shiga样毒素2的基因的核苷酸序列来自 e的亚基.已报告Coli （Genbank： ab035143 ）.
NR-45117未经确认功能活动释放.据报道，通过杂交瘤克隆11e10产生的单克隆抗体与重组1反应. Coli 滋贺皮
毒素2亚基A由ELISA和Western Blot.
每个小瓶NR-45117在PBS中含有大约100μL纯化的单克隆抗体，pH7.4.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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