- 4 次围观
产品图片
产品别名
货号/SKU
EKC006
货号/规格
1 vial
库存与交货期
1-2周
人民币价格
22600
试剂海关审批
使用人负责A/B风险申请资质
国外采购
支持/部分需签MTA
厂牌
Kerafast, Inc.
品牌
产品基础信息
From the laboratory of Lynda F. Bonewald, PhD, University of Missouri - Kansas City.
产品描述信息
Product Type: | Cell Line |
Name: | IDG-CM6 |
Cell Type: | Late cementoblast/cementocyte |
Organism: | Mouse |
Morphology: | Adherent, similar to an osteoblast/cementoblast-like cell which, under osteogenic conditions, can form a mineralized matrix, differentiate into an cementocyte-like cell, and express GFP driven by the Dentin Matrix Protein 1 promoter. |
Species: | Mouse |
Source: | Apical portion of tooth roots from 1st to 3rd mandibular molars |
Biosafety Level: | BSL 1 |
Subculturing: | Under permissive conditions at 33C, when at 80-90% confluent, use a 0.05% Trypsin/ 0.53mM EDTA solution to detach cells. Split 1:5 to 1:10 based on your need. Cells grow best when cultured at higher densities; at low densities, proliferation can be slow. Exchange media every 2-3 days. |
Growth Conditions: |
Proliferation medium: AlphaMEM (containing L-glutamine and deoxyribonucleosides); supplemented with 10% FBS; penicillin-streptomycin at 100U/ml-100ug/ml; Recombinant Mouse Interferon-gamma (INF-g) range 8-50 U/ml; for growing at 33°C. Differentiation medium: AlphaMEM (L-glutamine and deoxyribonucleosides); supplemented with 10% Fetal Bovine Serum; penicillin-streptomycin at 100U/ml-100ug/ml; approximately 50µg/ml Ascorbic Acid and 4mM β-glycerophosphate; for growing at 37°C. Grown on dishes coated with [0.15 mg/ml] rat tail type I collagen. |
Cryopreservation: | 60% AlphaMEM, 30% FBS, 10% DMSO, at 1-2 x 10^6 cells/vial/1ml |
Comments: | For experimental purposes, plate the cells at a density of 4x10^4 cells/cm2, in proliferation medium and incubate at 33C, 5% CO2 , until confluent, called Day 0. At Day 0, switch to differentiation medium, and incubate at 37°C, 5% CO2, exchanging media every 2-3 days. The GFP expression, controlled by the DMP1 promoter, is mildly observed at 5-7 days of culture under differentiation conditions, and usually peaks around day 35 of culture |
Storage: | After slowly freezing cryovials in a -80C freezer overnight, transfer to a liquid nitrogen tank for long term storage |
Shipped: | Dry Ice |
产品安全信息
Zhao N, Nociti Jr FH, Duan P, Prideaux M, Zhao H, Foster BL, Somerman MJ, Bonewald LF. Isolation and Functional Analysis of an Immortalized Murine Cementocyte Cell Line, IDG-CM6. J Bone Miner Res. 2016 Feb: 31(2): 430-442. DOI: 10.1002/jbmr.2690Zhao N, Foster BL, Bonewald LF. The Cementocyte-An Osteocyte Relative? J Dental Res. 2016, Vol 95 (7) 734-741. DOI: 10.1177/0022034516641898Woo SM, Rosser J, Dusevich V, Kalajzic I, Bonewald LF. Cell line IDG-SW3 replicates osteoblast-to-late-osteocyte differentiation in vitro and accelerates bone formation in vivo. J Bone Miner Res. 2011 Nov; 26(11):2634-46. DOI: 10.1002/jbmr.465If you publish research with this product, please let us know so we can cite your paper.
主要内容
克隆杂质细胞样细胞系,IDG-CM6重复腔内细胞,以晚期渗碳细胞分化。亮点:可以在IFN-γ存在下在33°C下在33°C下扩展,然后在没有IFN-γDMP1-GFP阴性和T-抗原的情况下在37℃下恢复其原始表型。 - 在永生化条件下,但DMP1-GFP阳性和T-抗原阴性在成骨细胞等骨质形成条件下,它们表达碱性磷酸酶并将一种I型胶原基质产生和矿化,如早期骨细胞,它们表达E11 / GP38 / Podoplanin和DMP -1如晚骨细胞,它们开发了树突状形态,表达SOST / SOSTOSTHE克隆杂质细胞样细胞系IDG-CM6(对于不朽的鼠/ DMP1-GFP-GFP-GFP-CM6),衍生自第1至第3个下颌摩尔齿根的顶端部分从3莫。携带DMP-1(牙本质基质蛋白-1)启动子的老雌性小鼠驱动GFP(绿色荧光蛋白)与不置牛排交叉,其表示通过IFN-γ(干扰素-γ)调节的Thermorabile SV40大肿瘤抗原。该细胞系应该有助于研究静晶细胞转变,生物抗细胞液体细胞功能的机制和SOST / SCLEROSTIN和DMP-1.从密苏里大学Lynda F. Bonewald,博士学位的实验室进行研究。
厂牌介绍
关于Kerafast Inc.
Kerafast 是一家位于波士顿的试剂公司,其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等,其中许多在其他地方无法获得。自 2011 年成立以来,来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂,无需通过传统的材料转让协议流程即可快速获取材料。
我们处理提供实验室的所有销售和运输物流,并从每次销售中返还丰厚的特许权使用费。因此,我们帮助提供实验室节省时间和资源,同时为进一步研究提供额外资金。采购科学家可以更轻松地发现和获取其他地方通常无法获得的独特试剂,同时还可以资助其他研究人员的工作。这创建了一个QuanQiu科学家社区,他们贡献和获取Reagent for the Greater Good,以加速他们自己的研究以及整体科学进步。
2018 年,Kerafast 与Absolute Antibody合并,后者是一家总部位于英国的公司,其愿景是为所有研究人员提供重组抗体技术。此次合并将两家公司聚集在一起,共同致力于改善科学界可用的研究工具的选择。
品牌标识
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