NR-51129_寨卡病毒、BeH819015、重组传染性克隆(人类/2015/巴西)(病毒）

NR-51129?? Zika virus, BeH819015, Recombinant Infectious Clone (Human/2015/Brazil)(Viruses)|Zika virus|Zika virus, BeH819015, Recombinant Infectious Clone (Human/2015/Brazil)|-60°C or colder|P Després, G GadeaAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Zika Virus, BeH819015, Recombinant Infectious Clone, NR-51129.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-51129 is a molecular clone of Zika virus (ZIKV), BeH819015. An infectious clone was assembled from four synthetic overlapping fragments corresponding to the full-length genomic RNA using the reverse genetic method designated ISA (Infectious-Subgenomic-Amplicons). The 5’-untranslated region (5’-UTR) is derived from clone MR766MC and the 3’-untranslated region (3’-UTR) from a contemporaneous clinical isolate of ZIKV. ZIKV, BeH819015 was isolated from the blood of a human in Belém, Pará State, Brazil on July 23, 2015. The complete genomic sequence of ZIKV, BeH819015 has been determined (GenBank: KU365778). Each vial contains approximately 1 mL of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (Vero 76, clone E6; ATCC ? CRL-1586?) infected with ZIKV, BeH819015, recombinant infectious clone.Additional information and tools are available at ViPR (Virus Pathogen Resource).