NR-49947金黄色葡萄球菌亚种.金黄色葡萄球菌,内布拉斯加州转座子突变库(NTML)遗传工具箱(突变细菌）

NR-49947??Staphylococcus aureus subsp. aureus, Nebraska Transposon Mutant Library (NTML) Genetic Toolbox(Mutant Bacteria)|Staphylococcus aureus subsp. aureus|Nebraska Transposon Mutant Library (NTML) Genetic Toolbox|-60°C or colder|K Bayles, P Fey, NARSAAcknowledgment for publications should read "The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: Nebraska Transposon Mutant Library (NTML) Genetic Toolbox, NR-49947."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.The Center for Staphylococcal Research (CSR) at the University of Nebraska Medical Center has generated the Nebraska Transposon Mutant Library (NTML), a collection of sequence-defined transposon (Tn) insertion mutants of Staphylococcus aureus (S. aureus). To increase the functionality of the NTML, an allelic exchange system, the NTML Genetic Toolbox, was developed for the easy exchange of the transposons with either selectable markers or promoterless reporter genes. The selectable markers can be used to create multiple defined mutations within the S. aureus chromosome; whereas the reporter genes allow for the generation of single copy reporter constructs within any gene included in the NTML. The exchange plasmids are comprised of either a selectable marker or a reporter gene, flanked by the 5' and 3' ends of the bursa aurealis Tn.The genetic toolbox contains all of the plasmids listed in Table 1 on the Product Information Sheet, transformed into S. aureus, strain RN4220 except for pJB38 which was transformed into Escherichia coli (E. coli), strain DH5a. pJB38 is the temperature-sensitive allelic exchange plasmid that is the parent plasmid to the toolbox's plasmid constructs and is available as BEI Resources NR-49932. The complete sequences of the plasmids have been determined and are available in the Additional Information section below. The complete plasmid sequences have been submitted to GenBank. The Nebraska Transposon Mutant Library (NTML) was constructed in the laboratories of Dr. Ken Bayles and Dr. Paul Fey at the University of Nebraska Medical Center. Additional information is available at the NTML website. Each tube of recombinant S. aureus contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth with 10 ?g/mL chloramphenicol supplemented with 25% glycerol. The recombinant E. coli tube contains approximately 0.5 mL of bacterial culture in Luria-Bertani (LB) broth with 100 ?g/mL ampicillin supplemented with 25% glycerol.