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产品图片
货号/SKU
NR-48501
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、2
产品描述信息
NR-48501??Staphylococcus aureus subsp. aureus, Nebraska Transposon Mutant Library (NTML) Screening Array(Mutant Bacteria)|Staphylococcus aureus subsp. aureus|Nebraska Transposon Mutant Library (NTML) Screening Array|-60°C or colder|K Bayles, P Fey, NARSAAcknowledgment for publications should read "The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution through BEI Resources, NIAID, NIH: Nebraska Transposon Mutant Library (NTML) Screening Array, NR-48501."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
The NTML (NTML) Screening Array consists of approximately 2000 Staphylococcus aureus (S. aureus) subsp. aureus USA300 JE2, transposon (Tn) mutants arrayed in five 384-well microtiter plates. The screening array was developed to be a resource for performing high-throughput phenotypic screens to identify candidate genes for future research. The mutants were derived from S. aureus subsp. aureus, strain JE2. Mutagenesis occurred through the use of the mariner-based transposon bursa aurealis resulting in erythromycin resistant deletion strains of JE2. The insertion sites were identified by sequencing the junction fragments containing the end of the transposon and the flanking DNA. The gene names and descriptions associated with each of the Tn mutants were obtained from the National Center for Biotechnology Information.
Note: Please refer to the attached spreadsheet in the additional information section of this website to determine the identity of the specific mutant in each well of each plate.
Each well contains approximately 150 ?l of bacterial culture in Tryptic Soy broth containing 5 ?g/mL erythromycin supplemented with 25% glycerol.
The Nebraska Transposon Mutant Library (NTML) was constructed in the laboratories of Dr. Ken Bayles and Dr. Paul Fey at the University of Nebraska Medical Center. Additional information is available at the NTML website.
Note: Production in the 384-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual clone identities provided by the contributor.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
The NTML (NTML) Screening Array consists of approximately 2000 Staphylococcus aureus (S. aureus) subsp. aureus USA300 JE2, transposon (Tn) mutants arrayed in five 384-well microtiter plates. The screening array was developed to be a resource for performing high-throughput phenotypic screens to identify candidate genes for future research. The mutants were derived from S. aureus subsp. aureus, strain JE2. Mutagenesis occurred through the use of the mariner-based transposon bursa aurealis resulting in erythromycin resistant deletion strains of JE2. The insertion sites were identified by sequencing the junction fragments containing the end of the transposon and the flanking DNA. The gene names and descriptions associated with each of the Tn mutants were obtained from the National Center for Biotechnology Information.
Note: Please refer to the attached spreadsheet in the additional information section of this website to determine the identity of the specific mutant in each well of each plate.
Each well contains approximately 150 ?l of bacterial culture in Tryptic Soy broth containing 5 ?g/mL erythromycin supplemented with 25% glycerol.
The Nebraska Transposon Mutant Library (NTML) was constructed in the laboratories of Dr. Ken Bayles and Dr. Paul Fey at the University of Nebraska Medical Center. Additional information is available at the NTML website.
Note: Production in the 384-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual clone identities provided by the contributor.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送至NIAID进行批准.
此项目目前处于我们的生产队列中.请允许充足的时间进行分销批次.
NTML（NTML）筛选阵列由大约2000℃的金黄色葡萄球菌（A. aureus）（S. aureus）子公司组成. 金黄色葡萄球菌 USA300 JE2，转座子（TN）突变体排列在五个384孔微量滴定板中.筛选阵列被开发为用于进行高通量表型筛网的资源，以识别未来研究的候选基因.突变体衍生自.金黄色葡萄球菌 subsp. 金黄色葡萄球菌，菌株JE2.诱变通过使用水手而被捕获的转座子 Bursa Aurealis 导致JE2的红霉素耐药菌株.通过测序含有转座子末端和侧翼DNA的结碎片来鉴定插入位点.与每个TN突变体相关的基因名称和描述是从国家生物技术信息中心获得的.
注意：请参阅本网站附加信息部分中的附加电子表格，以确定每个板上每个孔的特定突变体的身份.
每个孔中含有大约150μl的细菌培养物，含有5μg/ ml补充有25％甘油的红霉素的胰蛋白酶肉汤.
内布拉斯加州转座子突变体图书馆（NTML）是在Ken Bayles博士的实验室和内布拉斯加州大学医疗中心的Paul Fesy博士的实验室中构建. ntml 网站提供了附加信息.
注意：384孔格式的生产增加了相邻井之间的交叉污染的风险.应纯化各个克隆（例如，使用良好的微生物实践的单菌落分离和纯化）并在使用前进行序列验证. Bei资源不确认或验证贡献者提供的单个克隆身份.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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