NR-42012布氏锥虫亚种.brucei,Lister42729-13(TetRT7RNAP)(procyclicform)(寄生原生动物)

NR-42012??Trypanosoma brucei subsp. brucei, Lister 427 29-13 (TetR T7RNAP) (procyclic form)(Parasitic Protozoa)|Trypanosoma brucei subsp. brucei|Lister 427 29-13 (TetR T7RNAP) (procyclic form)|-130°C or colder (Note: Do not under any circumstances store vials at temperatures warmer than -70°C. Storage under these conditions will result in the death of the culture.)|G CrossAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Trypanosoma brucei, Strain Lister 427 29-13 (TetR T7RNAP) (procyclic form), NR-42012."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.Trypanosoma brucei (T. brucei) subsp. brucei, strain Lister 427 29-13 (TetR T7RNAP) was deposited to BEI Resources as a procyclic form cell line that expresses the T7 RNA polymerase (T7RNAP) and the tetracycline repressor (TetR) genes. Strain Lister 427 29-13 (TetR T7RNAP) was derived by sequential stable transfection of the procyclic form of wild-type T. brucei subsp. brucei, strain Lister 427 (BEI Resources NR-42010). The parent strain Lister 427 is a virulent lab strain isolated in 1960 from a sheep in Uganda and transferred to the Lister Institute in London in 1961.T. brucei subsp. brucei, strain Lister 427 29-13 (TetR T7RNAP), is a neomycin and hygromycin-resistant, transgenic procyclic form cell line, that co-expresses the T7RNAP and TetR.Each vial of NR-42012 contains approximately 0.5 mL of culture in cryopreservative.