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产品图片

货号/SKU
NR-40254
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-40254?? Monoclonal Anti-Crimean-Congo Hemorrhagic Fever Virus Pre-Gc Glycoprotein, Clone 12A9 (produced in vitro)(Monoclonal Antibodies)|Crimean-Congo hemorrhagic fever virus|Monoclonal Anti-Crimean-Congo Hemorrhagic Fever Virus Pre-Gc Glycoprotein, Clone 12A9 (produced in vitro)|-20°C or colder|CS SchmaljohnAcknowledgment for publications should read "The following reagent was obtained from the Joel M. Dalrymple - Clarence J. Peters USAMRIID Antibody Collection through BEI Resources, NIAID, NIH: Monoclonal Anti-Crimean-Congo Hemorrhagic Fever Virus Pre-Gc Glycoprotein, Clone 12A9 (produced in vitro), NR-40254."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Antibody Class: IgG1κ
Mouse monoclonal antibody prepared against the Crimean-Congo hemorrhagic fever virus (CCHFV) Pre-Gc glycoprotein was affinity purified from clone 12A9 hybridoma supernatant. Lot 61506733 was purified using protein G magnetic beads. Lot 70008695 was purified by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from BALB/c mice immunized with protein A sepharose-bound CCHFV glycoprotein-antibody complexes as described by Bertolotti-Ciarlet et al.
This reagent is part of the Joel M. Dalrymple - Clarence J. Peters USAMRIID Antibody Collection.
Each vial of NR-40254 contains approximately 100 ?L of purified monoclonal antibody in PBS.
NR-40254 is reactive in indirect immunofluorescence assays using Vero E6 cells infected with CCHFV, and neutralizes CCHFV in plaque reduction neutralization tests. Clone 12A9 antibody is also reported to function in ELISA and immunoprecipitation assays, to partially protect suckling mice from lethal CCHFV challenge, and to recognize an epitope that is conserved among some, but not all, CCHFV strains tested.
Antibody Class: IgG1κ
Mouse monoclonal antibody prepared against the Crimean-Congo hemorrhagic fever virus (CCHFV) Pre-Gc glycoprotein was affinity purified from clone 12A9 hybridoma supernatant. Lot 61506733 was purified using protein G magnetic beads. Lot 70008695 was purified by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from BALB/c mice immunized with protein A sepharose-bound CCHFV glycoprotein-antibody complexes as described by Bertolotti-Ciarlet et al.
This reagent is part of the Joel M. Dalrymple - Clarence J. Peters USAMRIID Antibody Collection.
Each vial of NR-40254 contains approximately 100 ?L of purified monoclonal antibody in PBS.
NR-40254 is reactive in indirect immunofluorescence assays using Vero E6 cells infected with CCHFV, and neutralizes CCHFV in plaque reduction neutralization tests. Clone 12A9 antibody is also reported to function in ELISA and immunoprecipitation assays, to partially protect suckling mice from lethal CCHFV challenge, and to recognize an epitope that is conserved among some, but not all, CCHFV strains tested.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送至NIAID进行批准. 抗体类:IgG1κ小鼠单克隆
针对克里米尔刚果出血热病毒(CCHFV)前GC的抗体
糖蛋白是从克隆12a9杂交瘤上清液中纯化的亲和力.很多
使用蛋白G磁性纯化61506733
珠子.批量纯化了70008695
蛋白G亲和层析.这
B细胞杂交瘤由SP2 / 0-AG14小鼠骨髓瘤细胞的融合产生
来自Balb / c小鼠的脾细胞用蛋白质免疫琼脂糖结合的CCHFV
糖蛋白 - 抗体复合物如Bertolotti-Ciarlet等.在PBS中含有大约100μl纯化的单克隆抗体. NR-40254使用CCHFV感染的Vero E6细胞在间接免疫荧光测定中是反应性的,并在斑块还原中和CCHFV中和CCHFV.还报道克隆12A9抗体在ELISA和免疫沉淀测定中起作用,以部分保护来自致命的CCHFV攻击的乳胶小鼠,并识别在某些情况下保守的表位,但并非所有的CCHFV菌株.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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