- 6 次围观
产品图片
货号/SKU
NR-36463
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-36463??Human respiratory syncytial virus , Respiratory Syncytial Virus (RSV) A2 Phosphoprotein (P) Helper Plasmid, pA2-Popt(Plasmid/Vectors)|Human respiratory syncytial virus|Respiratory Syncytial Virus (RSV) A2 Phosphoprotein (P) Helper Plasmid, pA2-Popt|-80°C or colder|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Respiratory Syncytial Virus (RSV) A2 Phosphoprotein (P) Helper Plasmid, pA2-Popt, NR-36463."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-36463 is a component of a bacterial artificial chromosome (BAC)-based RSV rescue system that allows RSV infection to be monitored by fluorescence and is an important tool in RSV vaccine research and mutagenesis studies.
The P helper plasmid was constructed from codon-optimized RSV A2 P sequences. The codon-optimized cDNA sequences were synthesized and cloned into the pcDNA? 3.1(+) mammalian expression plasmid (Life Technologies? Invitrogen?). The plasmid was produced in Escherichia coli, strain 10-beta (a DH10B derivative, New England BioLabs?) and extracted using a Endo-Free Plasmid Maxi Kit (Qiagen).
Each vial contains 0.5 ?g of plasmid DNA in RNase/DNase-free 10 mM Tris-HCl, 1 mM EDTA buffer (pH 8). The vial should be centrifuged prior to opening.
Recombinant RSV was produced by co-transfection of BHK-21 clone BSR T7/5 cells with pSynkRSV-I19F, a BAC plasmid containing RSV A2-line19F antigenomic DNA and the gene for the far-red fluorescent protein monomeric Katushka 2 (mKate2) to enable detection of infection through fluorescence, (NR-36460) and four helper plasmids encoding sequence-optimized genes from RSV strain A2: large polymerase (L) (NR-36461), nucleoprotein (N) (NR-36462), phosphoprotein (P) (NR-36463) and matrix 2-1 protein (M2-1) (NR-36464). RSV rescue and infection could be detected by red fluorescent syncytia.
NR-36463 is a component of a bacterial artificial chromosome (BAC)-based RSV rescue system that allows RSV infection to be monitored by fluorescence and is an important tool in RSV vaccine research and mutagenesis studies.
The P helper plasmid was constructed from codon-optimized RSV A2 P sequences. The codon-optimized cDNA sequences were synthesized and cloned into the pcDNA? 3.1(+) mammalian expression plasmid (Life Technologies? Invitrogen?). The plasmid was produced in Escherichia coli, strain 10-beta (a DH10B derivative, New England BioLabs?) and extracted using a Endo-Free Plasmid Maxi Kit (Qiagen).
Each vial contains 0.5 ?g of plasmid DNA in RNase/DNase-free 10 mM Tris-HCl, 1 mM EDTA buffer (pH 8). The vial should be centrifuged prior to opening.
Recombinant RSV was produced by co-transfection of BHK-21 clone BSR T7/5 cells with pSynkRSV-I19F, a BAC plasmid containing RSV A2-line19F antigenomic DNA and the gene for the far-red fluorescent protein monomeric Katushka 2 (mKate2) to enable detection of infection through fluorescence, (NR-36460) and four helper plasmids encoding sequence-optimized genes from RSV strain A2: large polymerase (L) (NR-36461), nucleoprotein (N) (NR-36462), phosphoprotein (P) (NR-36463) and matrix 2-1 protein (M2-1) (NR-36464). RSV rescue and infection could be detected by red fluorescent syncytia.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. NR-36463是基于RSV救援系统的细菌人工染色体（BAC）的组成部分，允许监控RSV感染通过荧光，是RSV疫苗研究和诱变研究中的重要工具. （+）哺乳动物表达质粒（Life Technologies?Invitrogen TM）中.在大肠杆菌中产生质粒，菌株10-β（DH10B衍生物，新英格兰Biolabs ?），并使用无源性质粒MAXI试剂盒（QIAGEN）提取. 每个小瓶在RNase / DNase的10mM Tris-HCl中含有0.5μg质粒DNA，1mM EDTA缓冲液（pH8）.在打开之前应该离心. 重组RSV是通过使用PsynkRSV-I19F的BHK-21克隆BSR T7 / 5细胞的共转染而产生的，含有RSV A2-Line19F Antigenomic DNA的BAC质粒和用于远红荧光蛋白单体Katushka 2（MKate2）的基因，以使得通过荧光（NR-36460）和四辅助质粒从RSV菌株A2（NR-36461）编码序列优化基因的序列优化基因的感染检测.（NR-36461 ），核蛋白（N）（NR-36462），磷蛋白（P）（NR-36463）和基质2-1蛋白（M2-1）（NR-36464）. RSV救援和感染可以通过红色荧光异性Syncytia检测.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
品牌标识
可能感兴趣的内容
2022-06-17
2022-06-17
2021-12-02
2022-03-22
2021-12-02
2022-04-01
2021-12-21
2025-03-18
2024-01-21
2021-10-31
2021-12-02
2021-12-21
2022-04-01