NR-28664甲型流感病毒,A/Wilson-Smith/1933-小鼠适应型(H1N1)(克隆)(病毒）

NR-28664?? Influenza A Virus, A/Wilson-Smith/1933-Mouse Adapted (H1N1) (clone)(Viruses)|Influenza A virus|Influenza A Virus, A/Wilson-Smith/1933-Mouse Adapted (H1N1) (clone)|-60°C or colder|EG BrownAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Influenza A Virus, A/Wilson-Smith/1933-Mouse Adapted (H1N1) (clone), NR-28664."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.Influenza A virus, A/Wilson-Smith/1933 (H1N1) was isolated from a human in the United Kingdom on April 16, 1933.Sequence information is available for influenza A virus, A/United Kingdom/1-MA/1933 (H1N1) at the Influenza Research Database. Influenza A virus, A/Wilson-Smith/1933 (H1N1) was cloned by limiting dilution at the Laboratory Center for Disease Control, Health Canada, Ottawa, and passaged twice in eggs. The cloned virus (available as BEI Resources NR-28663) was then passaged twelve times in mouse lung. A clonal isolate was obtained by two plaque purifications in MDCK cells and subsequently passaged twice in specific pathogen free embryonated chicken eggs before deposit to BEI Resources. Note that although NR-28664 was deposited to BEI Resources as A/Wilson-Smith/1933-mouse adapted (H1N1), nucleotide sequence obtained from the same source material by the NIAID Influenza Genome Sequencing Consortium was deposited to NCBI and IRD as A/United Kingdom/1-MA/1933 (H1N1). This simply reflects a renaming of the original Wilson-Smith isolate using the modern standard nomenclature for influenza strains, specifying the geographical location of isolation and a sequential isolate number, with the addition of the MA notation to indicate adaptation to the murine host.Each vial contains approximately 1 mL of pooled allantoic fluid from specific pathogen free (SPF) embryonated chicken eggs infected with influenza A virus, A/Wilson-Smith/1933-mouse adapted (H1N1).