产品图片
货号/SKU
NR-12132
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-12132??Bacillus anthracis, Exosporium Basal Layer Protein BxpB (Locus_Tag: BA_1237) with N-Terminal Histidine Tag, Recombinant from Escherichia coli(Proteins)|Bacillus anthracis|Exosporium Basal Layer Protein BxpB (Locus_Tag: BA_1237) with N-Terminal Histidine Tag, Recombinant from Escherichia coli|-80°C or colder|AD O'Brien, JF SinclairAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Bacillus anthracis Exosporium Basal Layer Protein BxpB (Locus_Tag: BA_1237) with N-Terminal Histidine Tag, Recombinant from Escherichia coli, NR-12132."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-12132 is a recombinant form of the Bacillus anthracis (B. anthracis) protein BxpB (locus_tag: BA_1237) which is known to be expressed in the exosporium and localized to the exosporium basal surface. The amino acid sequence includes 1) an N-terminal hexa-histidine tag 2) a thrombin cleavage site and 3) amino acid residues 1 to 167 of hypothetical protein BxpB from the Ames strain of B. anthracis (GenPept: AAP25195). The recombinant protein was expressed in Escherichia coli and purified by nickel affinity chromatography. NR-12132 has a theoretical molecular weight of approximately 19 kilodaltons.
NR-12132 reacts with rabbit polyclonal antibody to B. anthracis exosporium basal layer protein BxpB (BEI Resources NR-12133) as shown by western blot analysis.
Each vial contains approximately 10 ?g of NR-12132 in PBS.
NR-12132 is a recombinant form of the Bacillus anthracis (B. anthracis) protein BxpB (locus_tag: BA_1237) which is known to be expressed in the exosporium and localized to the exosporium basal surface. The amino acid sequence includes 1) an N-terminal hexa-histidine tag 2) a thrombin cleavage site and 3) amino acid residues 1 to 167 of hypothetical protein BxpB from the Ames strain of B. anthracis (GenPept: AAP25195). The recombinant protein was expressed in Escherichia coli and purified by nickel affinity chromatography. NR-12132 has a theoretical molecular weight of approximately 19 kilodaltons.
NR-12132 reacts with rabbit polyclonal antibody to B. anthracis exosporium basal layer protein BxpB (BEI Resources NR-12133) as shown by western blot analysis.
Each vial contains approximately 10 ?g of NR-12132 in PBS.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. NR-12132是 Bacillus炭疽(B.炭疽)蛋白质Bxpb()的重组形式Locus_tag:Ba_1237)已知在外孢菌中表达并局部地置于外孢子基表面.氨基酸序列包括1)n-末端六胞组氨酸标签2)凝血酶裂解位点和3)氨基酸残基从ames菌株的 b的假设蛋白质bxpb的氨基酸残基1-167.炭疽病(Genpept:AAP25195).重组蛋白在大肠杆菌中表达并通过镍亲和层析纯化. NR-12132具有大约19千伏的理论分子量. NR-12132与兔多克隆抗体反应 b.炭疽基底层蛋白质BXPB(BEI资源NR-12133),如Western印迹分析所示. 每个小瓶在PBS中含有大约10μg的NR-12132.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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