(类似于 VMC-31）

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Antibody Class: IgG1κ

Mouse monoclonal antibody to a recombinant form of the B5R envelope glycoprotein [B5R(275t); residues 20 to 275 comprising the ectodomain, N-terminal histidine-tagged] of the Western Reserve (WR) strain of vaccinia virus was purified from hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of SP2/0 myeloma cells with immunized BALB/c splenocytes.

Each vial contains approximately 100 ?L of purified monoclonal antibody in phosphate-buffered saline (PBS).

NR-51207 was purified from the same hybridoma as VMC-31. The specificity of VMC-31 was determined by reactivity to B5R(275t) by ELISA and confirmed by Western blot analysis under reducing and non-reducing conditions. The reactivity pattern in ELISA assays using overlapping peptides spanning residues 20 to 275 of B5R indicates that VMC-31 recognizes an epitope within amino acids 65 to 75. VMC-31 does not neutralize the infectivity of the extracellular enveloped virus (EEV) form of vaccinia virus in BSC-1 cells using an EEV plaque reduction assay. VMC-31 inhibits the comet tail formation of the EEV form of vaccinia virus in BSC-1 cells using a comet tail inhibition assay.