产品描述信息
NR-44229??Human respiratory syncytial virus , Genomic RNA from Human Respiratory Syncytial Virus, A1998/3-2(Nucleic Acids)|Human respiratory syncytial virus|Genomic RNA from Human Respiratory Syncytial Virus, A1998/3-2|-80°C or colder|ML MooreAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic RNA from Human Respiratory Syncytial Virus, A1998/3-2, NR-44229."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.This item is currently in our production queue. Please allow ample time for distribution lots to be made available. Genomic RNA was isolated from a preparation of cell lysate and supernatant from HEp-2 cells (ATCC ? CCL-23?) infected with Human Respiratory Syncytial Virus, A1998/3-2. Human respiratory syncytial virus (RSV), A1998/3-2 was isolated from a nasal wash from an infant with RSV bronchiolitis in Nashville, Tennessee on March 2, 1998. A1998/3-2 is one of six clinical RSV isolates that recently were shown to induce variable disease severity, lung interleukin-13 (IL-13) levels, and gob-5 levels in BALB/cJ mice.2 IL-13 is a cytokine linked to mucus production and gob-5 is a calcium-activated chloride channel family member implicated in airway inflammation. Compared to mock infection, RSV A1998/3-2 infection led to low levels of gob-5 in lung tissue, no significant elevation in IL-13 expression, and no weight loss in infected mice.NR-44229 has been qualified for PCR applications by amplification of an approximately 900 nucleotide sequence.Each vial contains 100 ?L of viral genomic RNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0). The viral genomic RNA is in a background of cellular nucleic acid and carrier RNA. The vial should be centrifuged prior to opening.