NR-45832Synfluenza(SyntheticInfluenza)CloneSet,RecombinantinEscherichiacoli,Plate14(Neuraminidase)(Clones)
产品图片
货号/SKU
NR-45832
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-45832?? Synfluenza (Synthetic Influenza) Clone Set, Recombinant in Escherichia coli, Plate 14 (Neuraminidase)(Clones)|Influenza A virus|Synfluenza (Synthetic Influenza) Clone Set, Recombinant in Escherichia coli, Plate 14 (Neuraminidase)|-60°C or colder|Pathogen Functional Genomics Resource Center at the J. Craig Venter InstituteAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Synfluenza (Synthetic Influenza) Clone Set, Recombinant in Escherichia coli, Plate 14 (Neuraminidase), NR-45832."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
The Synfluenza clone set is part of a National Institute of Allergy and Infectious Diseases (NIAID) initiative to create 1000 influenza gene segment clones from 12 host subtypes that span the protein sequence diversity of influenza viruses between 2005 and 2010. Each clone is designed from GenBank sequences with consensus untranslated regions. The purpose of the project is to develop the ability to create and stockpile synthetic DNA encoding influenza gene segments. These segments can then be used to generate virus seed stocks and a library of clones for vaccine, diagnostic and basic research.
The NIAID Genome Sequencing Center at the J. Craig Venter Institute constructed synthetic influenza neuraminidase (NA) and hemagglutinin (HA) genes using automated DNA synthesis and assembly. There are nine synthetic NA influenza clone plates (BEI numbers NR-45827 through NR-45833, NR-45090 and NR-45091) and six synthetic HA influenza clone plates (BEI numbers NR-45092 through NR-45097) in the set.
Each synthetic NA gene from NR-45832 was manufactured from five individually-designed, double-stranded DNA construct cassettes produced by assembly of eight chemically-synthesized oligonucleotides using the Gibson Assembly? process. The five cassettes were combined into the pSMART?-LCKan vector (Lucigen?) to establish gene segment clones in One Shot? TOP10 competent (Invitrogen?) Escherichia coli (E. coli) cells.
Detailed information provided by JCVI for the complete Synfluenza Clone Set, including insert sequence, is available in the attached Synfluenza Master Clone List.
Each well of the 96-well plate contains approximately 200 ?L of E. coli culture in Yeast Extract Tryptone media containing 25 ?g/mL kanamycin supplemented with 10% glycerol.
Note: Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use.
The Synfluenza clone set is part of a National Institute of Allergy and Infectious Diseases (NIAID) initiative to create 1000 influenza gene segment clones from 12 host subtypes that span the protein sequence diversity of influenza viruses between 2005 and 2010. Each clone is designed from GenBank sequences with consensus untranslated regions. The purpose of the project is to develop the ability to create and stockpile synthetic DNA encoding influenza gene segments. These segments can then be used to generate virus seed stocks and a library of clones for vaccine, diagnostic and basic research.
The NIAID Genome Sequencing Center at the J. Craig Venter Institute constructed synthetic influenza neuraminidase (NA) and hemagglutinin (HA) genes using automated DNA synthesis and assembly. There are nine synthetic NA influenza clone plates (BEI numbers NR-45827 through NR-45833, NR-45090 and NR-45091) and six synthetic HA influenza clone plates (BEI numbers NR-45092 through NR-45097) in the set.
Each synthetic NA gene from NR-45832 was manufactured from five individually-designed, double-stranded DNA construct cassettes produced by assembly of eight chemically-synthesized oligonucleotides using the Gibson Assembly? process. The five cassettes were combined into the pSMART?-LCKan vector (Lucigen?) to establish gene segment clones in One Shot? TOP10 competent (Invitrogen?) Escherichia coli (E. coli) cells.
Detailed information provided by JCVI for the complete Synfluenza Clone Set, including insert sequence, is available in the attached Synfluenza Master Clone List.
Each well of the 96-well plate contains approximately 200 ?L of E. coli culture in Yeast Extract Tryptone media containing 25 ?g/mL kanamycin supplemented with 10% glycerol.
Note: Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将被发送到发货前的批准. 此项目目前在我们的生产队列中.＆nbsp;请允许有充分的时间进行分销批次. Synfluenza Clone Set是国家过敏和传染病（NIAID）倡议的一部分，从12开始创造1000个流感基因段克隆培养2005年至2010年间流感病毒蛋白质序列多样性的宿主亚型.每个克隆都是由Genbank序列的设计，不合适的区域.该项目的目的是制定制造编码流感基因段的合成DNA的能力.然后可以使用这些段来产生病毒种子股和克隆文库，用于疫苗，诊断和基础研究. J.Craig Venter Institute建造合成流感神经氨酸酶（NA）的NIAID基因组测序中心血凝素（HA）基因使用自动DNA合成和组装.含有九种合成Na流感克隆平板（BEI编号NR-45827至NR-45833，NR-45090和NR-45091）和六种合成的HA流感克隆平板（BEI编号NR-45092至NR-45097）. 来自NR-45832的每种合成NA基因由使用Gibson组装?工艺组装八种化学合成的寡核苷酸的五种单独设计的双链DNA构建盒制成.将五个盒式磁带组合成PSMART ? -Lckan载体（Lucigen ?），以在一个镜头中建立基因段克隆? top10 possent（Invitrogen ?）大肠杆菌（大肠杆菌）细胞. JCVI为完整的Synfluenza Clone集，包括插入序列，附加的Synfluenza Master Clone列表提供了详细信息. 96孔板的每个孔含有约200μl的 e. Coli 在酵母提取物中的培养蛋白培养蛋白培养基，其含有25μg/ ml卡那霉素，其补充有10％甘油. 注意：96孔格式的生产增加了风险增加相邻孔之间的交叉污染.应纯化各个克隆（例如，使用良好的微生物学实践的单落区分离和纯化）和在使用前进行序列验证.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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