pfN 疟疾表达载体(质粒/载体），NR-1_Vaccinia virus

MRA-461??Plasmodium falciparum, pfN Malaria Expression Vector(Plasmid/Vectors)|Plasmodium falciparum|pfN Malaria Expression Vector|Room temperature, -20°C or colder once re-hydrated|CV PloweAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: pfN Malaria Expression Vector, MRA-461, contributed by Christopher V. Plowe."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material.pfN is a malaria expression vector with a geneticin/kanamycin-selectable marker for growth in either yeast or bacteria, respectively, while selection in malaria is governed by a neo under control of malaria regulatory sequences. A multiple-cloning site between an additional malaria regulatoy cassette allows for expression of a gene of interest after cloning in either yeast and bacteria.Crabb, B. S., et al. "Stable Transgene Expression in Plasmodium falciparum." Mol. Biochem. Parasitol. 90 (1997): 131-144. PubMed: 9497038.Mamoun, C. B., et al. "A Set of Independent Selectable Markers for Transfection of the Human Malaria Parasite Plasmodium falciparum." Proc. Natl. Acad. Sci. USA 96 (1999): 8716-8720. PubMed: 10411941.Wach, A. "PCR-Synthesis of Marker Cassettes with Long Flanking Homology Regions for Gene Disruptions in S. cerevisiae." Yeast 12 (1996): 259-265. PubMed: 8904338.