NR-853蓖麻,带有N末端组氨酸标签的蓖麻毒素A亚基,来自大肠杆菌的重组体(毒素)
产品图片
货号/SKU
NR-853
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-853??Ricinus communis, Ricin Toxin A Subunit with N-Terminal Histidine Tag, Recombinant from Escherichia coli(Toxins)|Ricinus communis|Ricin Toxin A Subunit with N-Terminal Histidine Tag, Recombinant from Escherichia coli|-20°C or colder|AD O'Brien, JF SinclairAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Ricin Toxin A Subunit with N-Terminal Histidine Tag, Recombinant from Escherichia coli, NR-853."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The sequence of the Ricinus communis gene for the ricin toxin precursor protein has been reported (GenBank: X03179).
NR-853 is a recombinant form of the A subunit of ricin toxin. The amino acid sequence includes an N-terminal histidine tag (MRGSHHHHHHTDPM) and amino acid residues 36 to 302 of the ricin toxin precursor. A QIAGEN pQE-32 vector was used to express the recombinant protein in Escherichia coli. The protein was purified by nickel affinity chromatography. NR-853 has a theoretical molecular weight of approximately 31,636 daltons.
NR-853 reacts specifically with polyclonal antibody to ricin holotoxin (BEI Resources NR-862), polyclonal antibody to ricin A chain (BEI Resources NR-863), and monoclonal antibody to ricin A chain (BEI Resources NR-843) as determined by Western blot analysis. NR-853 is not active in an in vitro cytotoxicity assay using Vero cells.
Each vial of NR-853 contains approximately 1 mg of recombinant ricin toxin A chain suspended in 10 mM sodium acetate buffer (pH 5.6).
The sequence of the Ricinus communis gene for the ricin toxin precursor protein has been reported (GenBank: X03179).
NR-853 is a recombinant form of the A subunit of ricin toxin. The amino acid sequence includes an N-terminal histidine tag (MRGSHHHHHHTDPM) and amino acid residues 36 to 302 of the ricin toxin precursor. A QIAGEN pQE-32 vector was used to express the recombinant protein in Escherichia coli. The protein was purified by nickel affinity chromatography. NR-853 has a theoretical molecular weight of approximately 31,636 daltons.
NR-853 reacts specifically with polyclonal antibody to ricin holotoxin (BEI Resources NR-862), polyclonal antibody to ricin A chain (BEI Resources NR-863), and monoclonal antibody to ricin A chain (BEI Resources NR-843) as determined by Western blot analysis. NR-853 is not active in an in vitro cytotoxicity assay using Vero cells.
Each vial of NR-853 contains approximately 1 mg of recombinant ricin toxin A chain suspended in 10 mM sodium acetate buffer (pH 5.6).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. ricinus communis 基因的序列(Genbank: X03179). NR-853是蓖麻毒素的亚基的重组形式.氨基酸序列包括N-末端组氨酸标签(MRGSHHHHHHHTDPM)和蓖麻毒素前体的氨基酸残基36至302. QIAGENPQE-32载体用于表达在大肠杆菌中的重组蛋白.通过镍亲和层析纯化蛋白质. NR-853具有约31,636道尔顿的理论分子量. 关于蓖麻素全洛蛋白(bei resource nr-862)的多克隆抗体,多克隆抗体对蓖麻链(BEI资源NR-863 ),并通过Western印迹分析测定的蓖麻辐射链(BEI资源NR-843)的单克隆抗体.使用VERO细胞,NR-853在体外的体外毒性测定中不活跃. 每个小瓶NR-853含有约1mg重组蓖麻毒素毒素悬浮在10mM钠中的链条醋酸盐缓冲液(pH 5.6).
厂牌介绍
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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