NR-700SARS冠状病毒,3CL蛋白酶,大肠杆菌重组体(蛋白质）
产品图片
货号/SKU
NR-700
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-700??SARS coronavirus, 3CL Protease, Recombinant from E. coli(Proteins)|SARS coronavirus|3CL Protease, Recombinant from E. coli|-20°C or colder|NIH Biodefense and Emerging Infections Research Resources RepositoryAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-CoV 3CL Protease, Recombinant from E. coli, NR-700."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-700 was expressed and purified using a novel SUMO fusion system. An N-terminal histidine-tagged SUMO-3CL protease fusion was expressed in E. coli and purified by nickel affinity chromatography. After the fusion was cleaved by the SUMO protease, the SUMO tag and the SUMO protease (both histidine-tagged) were subtracted from the 3CL protease by nickel affinity chromatography. The 3CL protease was further purified by anion exchange chromatography, dialyzed against 10 mM ammonium bicarbonate, aliquoted and lyophilized. NR-700 has a molecular weight of approximately 33,800 daltons. Using Western blot analysis, NR-700 reacted with rabbit polyclonal sera prepared against the SUMO 3CL protease fusion, but did not react with rabbit polyclonal sera prepared against a SUMO nucleocapsid fusion.
Each vial contains 1.0 mg of NR-700 lyophilized in 10 mM ammonium bicarbonate.
NR-700 was expressed and purified using a novel SUMO fusion system. An N-terminal histidine-tagged SUMO-3CL protease fusion was expressed in E. coli and purified by nickel affinity chromatography. After the fusion was cleaved by the SUMO protease, the SUMO tag and the SUMO protease (both histidine-tagged) were subtracted from the 3CL protease by nickel affinity chromatography. The 3CL protease was further purified by anion exchange chromatography, dialyzed against 10 mM ammonium bicarbonate, aliquoted and lyophilized. NR-700 has a molecular weight of approximately 33,800 daltons. Using Western blot analysis, NR-700 reacted with rabbit polyclonal sera prepared against the SUMO 3CL protease fusion, but did not react with rabbit polyclonal sera prepared against a SUMO nucleocapsid fusion.
Each vial contains 1.0 mg of NR-700 lyophilized in 10 mM ammonium bicarbonate.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID以批准. 使用新的Sumo Fusion系统表示和净化NR-700.将N-末端组氨酸标记的SUMO-3CL蛋白酶融合在大肠杆菌中表达并通过镍亲和层析纯化.通过SUMO蛋白酶裂解融合后，通过镍亲和层析从3CL蛋白酶中减去SUMO标签和SUMO蛋白酶（两个组氨酸标记）.通过阴离子交换色谱法进一步纯化3CL蛋白酶，透析酸化10mM碳酸氢铵，等离等离去，冻干. NR-700的分子量约为33,800道尔顿.使用Western印迹分析，NR-700与对来自SUMO 3CL蛋白酶融合制备的兔多克隆血清反应，但未与针对SUMO核衣壳融合制备的兔多克隆血清反应. 每个小瓶含有1.0mg NR-700冻干，在10mM碳酸氢铵中冻干.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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