NR-56447来自SARS相关冠状病毒2的刺突糖蛋白(稳定）,B.1.1.529谱系(Omicron变体）,带有C末端组氨酸和Avi标签,来自HEK293细胞(蛋白质）的重组
产品图片
货号/SKU
NR-56447
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-56447?? Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, B.1.1.529 Lineage (Omicron Variant) with C-Terminal Histidine and Avi Tags, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, B.1.1.529 Lineage (Omicron Variant) with C-Terminal Histidine and Avi Tags, Recombinant from HEK293 Cells|-20°C or colder|BEI ResourcesAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, B.1.1.529 Lineage (Omicron Variant) with C-Terminal Histidine and Avi Tags, Recombinant from HEK293 Cells, NR-56447.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), B.1.1.529 lineage (Omicron variant) was produced in human embryonic kidney HEK293 cells and purified by immobilized metal affinity chromatography. NR-56447 lacks the signal sequence and contains 1193 residues (ectodomain) of the SARS-CoV-2 S glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RRAR→GSAS; residues 682 to 685) and KV→PP mutations (residues 986 and 987; wild type numbering), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site and C-terminal octa-histidine tag fused to an AviTag? BirA biotinylation acceptor sequence. NR-56447 includes A67V, delH69-V70, T95I, G142D, delV143-Y145, delN211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K and L981F mutations in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-56447 has a theoretical molecular weight of 139,900 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.46 ? resolution (PDB: 6VSB).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer and, when bound to a host receptor, allows fusion of the viral and cellular membranes. B.1.1.529 is one of several lineages and sublineages designated Omicron by the World Health Organization (WHO) and was first identified in South Africa, followed by multiple countries in November 2021. This lineage contains multiple mutations in the receptor-binding domain (RBD) that have been identified in other variants, including K417N, N501Y and D614G. The presence of D614G among variants has been shown to increase transmissibility, with K417N, Q493R, N501Y and Y505H shown to be important residues mediating virus entry into host cells. E484A has been shown to decrease neutralization by post-vaccination sera and some monoclonal antibody treatments.
Each vial contains approximately 100 microliters of NR-56447 in 10 mM HEPES, pH 7, 150 mM NaCl and 2 mM ethylenediamine-tetraacetic acid (EDTA).
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), B.1.1.529 lineage (Omicron variant) was produced in human embryonic kidney HEK293 cells and purified by immobilized metal affinity chromatography. NR-56447 lacks the signal sequence and contains 1193 residues (ectodomain) of the SARS-CoV-2 S glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RRAR→GSAS; residues 682 to 685) and KV→PP mutations (residues 986 and 987; wild type numbering), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site and C-terminal octa-histidine tag fused to an AviTag? BirA biotinylation acceptor sequence. NR-56447 includes A67V, delH69-V70, T95I, G142D, delV143-Y145, delN211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K and L981F mutations in the S glycoprotein as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). NR-56447 has a theoretical molecular weight of 139,900 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.46 ? resolution (PDB: 6VSB).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer and, when bound to a host receptor, allows fusion of the viral and cellular membranes. B.1.1.529 is one of several lineages and sublineages designated Omicron by the World Health Organization (WHO) and was first identified in South Africa, followed by multiple countries in November 2021. This lineage contains multiple mutations in the receptor-binding domain (RBD) that have been identified in other variants, including K417N, N501Y and D614G. The presence of D614G among variants has been shown to increase transmissibility, with K417N, Q493R, N501Y and Y505H shown to be important residues mediating virus entry into host cells. E484A has been shown to decrease neutralization by post-vaccination sera and some monoclonal antibody treatments.
Each vial contains approximately 100 microliters of NR-56447 in 10 mM HEPES, pH 7, 150 mM NaCl and 2 mM ethylenediamine-tetraacetic acid (EDTA).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
在人胚胎肾HEK293细胞中产生来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），B.1.1.529谱系（OMICRON变体）的重组形式.在人胚胎肾HEK293细胞中，并通过固定金属纯化亲和层析. NR-56447缺乏信号序列并含有1193个残基（Ectodomain）的SARS-COV-2 S糖蛋白;通过在Furin S1 / S2切割位点（RrAr→GSAs;残基682至685）和KV→PP突变（残留物986和987;野生型编号），并包括T4 Folcon三聚化结构域，HRV3C，通过取代重组蛋白质蛋白酶切割位点和C末端Octa-组氨酸标签与Avitag?Bira Biotinylation受体序列融合. NR-56447包括A67V，DELH69-V70，T95I，G142D，DELV143-Y145，DELN211，L212i，INS214EPE，G339D，S371L，S3733，S375F，K417N，N440K，G446S，S477N，T478K，E484A，Q493R，G496S，Q498R，G496S，Q498R，与SARS-COV-2参考序列（Genpept： qhd43416 ）. NR-56447具有139,900道尔顿的理论分子量. SARS-COV-2中三聚糖蛋白的晶体结构已经解决了3.46埃分辨率（PDB： 6VSB ）.
S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. B.1.1.529是世界卫生组织（世卫组织）的几个谱系和Sublineoges之一，并于11月2021年在南非首次确定.该谱系在受体结合域中含有多种突变（已在其他变体中鉴定的RBD，包括K417N，N501Y和D614G.已经显示出变体中D614G的存在，以增加透射率，K417N，Q493R，N501Y和Y505H所示是介导病毒进入宿主细胞的重要残留物. E484A已被证明通过疫苗接种后血清和一些单克隆抗体治疗来降低中和.
每个小瓶在10mM HEPES，pH 7,150mM NaCl和2mM乙二胺 - 四乙酸（EDTA）中含有大约100微升NR-56447.
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